{"title":"Detection of malachite green by electrochemical sensor constructed from modified high-affinity aptamer","authors":"Zanlin Chen, Haiming Li, Yian Chen, Shuangyan Han","doi":"10.1007/s00604-025-07506-1","DOIUrl":null,"url":null,"abstract":"<div><p>A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green (MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (K<sub>d</sub>) of 45.4 <span>\\(\\pm\\)</span> 17.6 μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower K<sub>d</sub> of 2.71 <span>\\(\\pm\\)</span> 1.95 μM. This sensor exhibited a limit of detection (LOD) value of 2.74 pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07506-1","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green (MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (Kd) of 45.4 \(\pm\) 17.6 μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower Kd of 2.71 \(\pm\) 1.95 μM. This sensor exhibited a limit of detection (LOD) value of 2.74 pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
