Screening, isolation and characterization of bioactive compound from ethanol extract of Maesa indica (Roxb.) Sweet leaf against HepG2 cancer cells

Pharmacological Research - Natural Products Pub Date : 2025-09-16 DOI:10.1016/j.prenap.2025.100376

K.P. Pooja , H.C. Shrishail , K.G. Aishwarya

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Abstract

Maesa indica (Roxb.) Sweet, is an evergreen shrub of the primulaceae family, is used traditionally for medicinal uses, including cancer treatment. However, phytochemical and bioactivity studies on its leaf extract remain limited. This study aims to analyze the phytochemical composition and identify bioactive compounds using LC-Q-TOF-MS, isolate and characterize a pure compound, and to evaluate its cytotoxic efficacy. To characterize the compound, we used HPLC, LCMS, NMR & FTIR techniques. In-vitro cytotoxicity of the leaf crude extract and the isolated compound was determined by MTT assay against the HepG2 cell line. The study revealed the presence of a wide range of phytochemicals. LC-Q-TOF-MS analysis confirmed the presence of 10 bioactive compounds. The isolated compound was identified as hesperetin. The MTT assay exhibited a concentration-dependent effect, with the ethanol extract showing an IC₅₀ value of 122.19 ± 0.44 µg/mL against HepG2 cells. Hesperetin exhibited moderate cytotoxic efficacy with an IC₅₀ value of 66.25 ± 0.96 µg/mL (p < 0.05). The results showed that leaf ethanol extract may contain effective compounds with a broad spectrum of curative applications. This report is the first one on the isolation of hesperetin and the characterization of a compound from the leaf ethanol extract of M. indica.

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黄花菜乙醇提取物生物活性化合物的筛选、分离与表征甜叶抗HepG2癌细胞

Maesa indica（英国）甜，是报春花科的常绿灌木，传统上用于医药用途，包括治疗癌症。然而，对其叶提取物的植物化学和生物活性研究仍然有限。本研究旨在利用LC-Q-TOF-MS分析其植物化学成分，鉴定其生物活性成分，分离纯化化合物，并对其细胞毒作用进行评价。为了对化合物进行表征，我们使用了HPLC、LCMS、NMR和FTIR技术。采用MTT法测定其对HepG2细胞株的体外细胞毒性。这项研究揭示了多种植物化学物质的存在。LC-Q-TOF-MS分析证实了10种生物活性化合物的存在。分离得到的化合物鉴定为橙皮苷。MTT实验显示浓度依赖效应，乙醇提取物对HepG2细胞的IC50值为122.19 ± 0.44 µg/mL。橙皮苷表现出中等的细胞毒作用，IC50值为66.25 ± 0.96 µg/mL （p <； 0.05）。结果表明，叶乙醇提取物可能含有具有广谱治疗应用的有效化合物。本文首次报道了从籼米叶乙醇提取物中分离得到的橙皮苷及其化合物的性质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pharmacological Research - Natural Products

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