A preliminary study of synovial fluid-derived mesenchymal stromal cell therapy for canine osteoarthritis

IF 1.8 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2025-09-15 DOI:10.1016/j.rvsc.2025.105897

Yasuhiro Koga , Makoto Fujiki , Tatsuro Hifumi , Erisa Moriwaki , Shunmei Chiba , Kazuhiro Misumi

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Abstract

Dogs with osteoarthritis (OA) may benefit from stromal cell therapies, but there is a lack of such established therapies. Accordingly, we investigated the feasibility and safety of synovial fluid (SF)-derived mesenchymal stromal cell (MSC) transplantation for canine OA. MSCs were isolated from SF (n = 15) or by joint lavage (JLF; n = 12) from the knee joints of dogs undergoing orthopedic surgery (n = 25), and then cultured on a dish (passage 1) and a polyethylene terephthalate (PET) nonwoven fabric scaffold (passage 2). We obtained 2166 ± 1177 (SF) or 1640 ± 1064 (JLF) × 10⁴ primary cells and 18,673 ± 8500 (SF) or 16,870 ± 3348 (JLF) × 10⁴ passaged cells, surpassing the target number. Passaged cells were morphologically uniform, self-proliferating, CD29, CD44, CD90, and CD105-positive, and CD31-negative cells, with the ability to differentiate into osteoblasts, chondrocytes, and adipocytes. Healthy dogs (n = 3) then received a 1-mL allogenic MSC suspension (at 1×, 5×, and 10 × 10⁷ cells), harvested and prepared with the same method, by intraarticular injection (with contralateral knees as negative controls), and were evaluated for adverse clinical signs, hematologic and biochemical parameters periodically, and joint images and pathology at day 90. No abnormal findings were found in any dogs, confirming the safety of allogeneic MSC transplantation

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滑膜液源性间充质细胞治疗犬骨关节炎的初步研究

患有骨关节炎（OA）的狗可能从基质细胞治疗中受益，但缺乏这种成熟的治疗方法。因此，我们研究了滑膜液来源的间充质间质细胞（MSC）移植治疗犬骨性关节炎的可行性和安全性。从骨科手术犬膝关节（n = 25）的SF （n = 15）或关节洗胃（JLF; n = 12）中分离MSCs，然后在培养皿（传代1）和聚对苯二甲酸乙二醇酯（PET）无纺布支架（传代2）上培养。我们获得2166±1177 （SF）或1640±1064 （JLF） × 104个原代细胞和18,673±8500 （SF）或16,870±3348 （JLF） × 104个传代细胞，超过了目标数量。传代细胞形态一致，自我增殖，CD29、CD44、CD90和cd105阳性，cd31阴性，具有向成骨细胞、软骨细胞和脂肪细胞分化的能力。健康犬（n = 3）接受1 ml同种异体间充质干细胞悬液（1×、5×和10 × 107细胞），以相同的方法采集并制备（对侧膝关节为阴性对照），定期评估不良临床体征、血液学和生化参数，并在第90天评估关节图像和病理。未发现任何异常结果，证实了同种异体间充质干细胞移植的安全性

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.