Phyllosphere microbiota and metabolomic insights of sewage sludge-derived biostimulant in enhancing nutritional quality and disease resistance of Pakchoi cabbage

Jiahou Hao , Jun Xu , Qiaoqiao Li , Zuoyue Li , Yue Zhang , Shuo Wang , Ji Li
{"title":"Phyllosphere microbiota and metabolomic insights of sewage sludge-derived biostimulant in enhancing nutritional quality and disease resistance of Pakchoi cabbage","authors":"Jiahou Hao ,&nbsp;Jun Xu ,&nbsp;Qiaoqiao Li ,&nbsp;Zuoyue Li ,&nbsp;Yue Zhang ,&nbsp;Shuo Wang ,&nbsp;Ji Li","doi":"10.1016/j.horiz.2025.100161","DOIUrl":null,"url":null,"abstract":"<div><div>Maintaining green and sustainable agriculture is challenging due to the reliance on chemical fertilizers and pesticides. Sewage sludge-based nutrient biostimulant (SS-NB), derived from alkaline thermal hydrolysis of sludge, offers both nutrient and biostimulant properties, making it a promising alternative. However, its effect on the physiological characteristics of crops remains unclear. In this study, we explored changes in the growth characteristics, metabolic molecules, and phyllosphere microbial communities of <em>pakchoi</em> cabbage following both foliar fertilization (FF) and soil fertilization (SF) with SS-NB. Both SS-NB(FF) and SS-NB(SF) treatments increased the net photosynthetic rate, light energy use efficiency, ATP synthase, and Rubisco enzyme activities in the photosynthesis system. The related metabolites were mainly involved in carbon fixation, sugar metabolism, amino acid metabolism, and disease-resistant metabolic pathways. In addition, SS-NB(SF) significantly enhanced the abundance of phytohormone analogues, which had strong positive correlations with photosynthesis and nutrient metabolites (up to 60.81%). Meanwhile, SS-NB(FF) and SS-NB(SF) reduced the crop's dependence on phyllosphere photosynthetic microorganisms and elevated the abundance of phyllosphere beneficial microorganisms. In the SS-NB(FF) group, bacteria acted as a hub for nutrient transformation and maintained strong interaction with metabolites, which in turn enhanced the disease resistance of the crop. Conversely, the SS-NB(SF) group suppressed phyllosphere pathogens through flavonoids in a negative interaction mode. Overall, SS-NB may enhance crop growth and disease resistance by inducing the production of phytohormonal substances and stimulating photosynthesis, nutrient metabolism, and flavonoid metabolic pathways. This study provides data support for SS-NB as a nutrient input to enhance sustainable agricultural production.</div></div>","PeriodicalId":101199,"journal":{"name":"Sustainable Horizons","volume":"16 ","pages":"Article 100161"},"PeriodicalIF":0.0000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sustainable Horizons","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772737825000318","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Maintaining green and sustainable agriculture is challenging due to the reliance on chemical fertilizers and pesticides. Sewage sludge-based nutrient biostimulant (SS-NB), derived from alkaline thermal hydrolysis of sludge, offers both nutrient and biostimulant properties, making it a promising alternative. However, its effect on the physiological characteristics of crops remains unclear. In this study, we explored changes in the growth characteristics, metabolic molecules, and phyllosphere microbial communities of pakchoi cabbage following both foliar fertilization (FF) and soil fertilization (SF) with SS-NB. Both SS-NB(FF) and SS-NB(SF) treatments increased the net photosynthetic rate, light energy use efficiency, ATP synthase, and Rubisco enzyme activities in the photosynthesis system. The related metabolites were mainly involved in carbon fixation, sugar metabolism, amino acid metabolism, and disease-resistant metabolic pathways. In addition, SS-NB(SF) significantly enhanced the abundance of phytohormone analogues, which had strong positive correlations with photosynthesis and nutrient metabolites (up to 60.81%). Meanwhile, SS-NB(FF) and SS-NB(SF) reduced the crop's dependence on phyllosphere photosynthetic microorganisms and elevated the abundance of phyllosphere beneficial microorganisms. In the SS-NB(FF) group, bacteria acted as a hub for nutrient transformation and maintained strong interaction with metabolites, which in turn enhanced the disease resistance of the crop. Conversely, the SS-NB(SF) group suppressed phyllosphere pathogens through flavonoids in a negative interaction mode. Overall, SS-NB may enhance crop growth and disease resistance by inducing the production of phytohormonal substances and stimulating photosynthesis, nutrient metabolism, and flavonoid metabolic pathways. This study provides data support for SS-NB as a nutrient input to enhance sustainable agricultural production.
污水污泥源生物刺激素提高小白菜营养品质和抗病性的根层微生物群和代谢组学研究
由于对化肥和农药的依赖,维持绿色和可持续农业具有挑战性。基于污水污泥的营养性生物刺激素(SS-NB)是由污泥的碱性热水解而来,具有营养和生物刺激素的双重特性,是一种很有前途的替代品。然而,其对作物生理特性的影响尚不清楚。本研究以小白菜为研究对象,探讨了叶片施肥(FF)和土壤施肥(SF)对小白菜生长特性、代谢分子和层际微生物群落的影响。SS-NB(FF)和SS-NB(SF)处理均提高了光合系统的净光合速率、光能利用效率、ATP合酶和Rubisco酶活性。相关代谢产物主要参与固碳、糖代谢、氨基酸代谢和抗病代谢途径。此外,SS-NB(SF)显著提高了植物激素类似物的丰度,其丰度与光合作用和养分代谢物呈强正相关(最高达60.81%)。同时,SS-NB(FF)和SS-NB(SF)降低了作物对叶层光合微生物的依赖,提高了叶层有益微生物的丰度。在SS-NB(FF)组中,细菌充当养分转化的枢纽,并与代谢物保持强烈的相互作用,从而增强了作物的抗病性。相反,SS-NB(SF)组通过黄酮类化合物以负互作模式抑制叶根球病原体。综上所述,SS-NB可能通过诱导植物激素物质的产生,刺激光合作用、养分代谢和类黄酮代谢途径来促进作物生长和抗病性。本研究为SS-NB作为促进农业可持续生产的养分投入提供了数据支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
6.60
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信