Genetic diversity of two races of Meloidogyne enterolobii affecting crops in Brazil and evaluation of new cultivars for host race testing

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-09-11 DOI:10.1016/j.pmpp.2025.102946

Caio Felipe de Barros Souza , Ana Luisa Porto Cruz , Vanessa S. Mattos , Sheila Freitas Almeida , Fabiano José Perina , Philippe Castagnone-Sereno , Juvenil Enrique Cares , Regina Maria Dechechi Gomes Carneiro

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引用次数: 0

Abstract

This study investigated the host races and genetic diversity of Brazilian populations of Meloidogyne enterolobii, an important nematode species with a wide host range that poses a major threat to tropical agriculture. The North Carolina Differential Hosts Test (NCDHT) demonstrated distinct pathogenic profiles in five Brazilian populations, supporting a subclassification of M. enterolobii into two physiological races: race 1 (positive reaction on tomato, tobacco, watermelon, and pepper, but not on cotton and peanut) and race 2 (also positive reaction on cotton, but not on peanut). Since the standard NCDHT cultivars are are not available in Brazil, we evaluated the compatibility of locally available cultivars as potential alternatives. The recommended cultivars for differential diagnosis are tomato ‘Santa Clara’, pepper ‘Magali R’, watermelon ‘Crimson sweet’, peanut ‘IAC Tatu’, tobacco ‘NC4’ and cotton ‘FM966’. Other cultivars with similar genetic backgrounds may also be suitable. Genetic variability was assessed using RAPD and AFLP primers. Low variability among M. enterolobii populations was detected. Concatenated neighbor-joining analysis grouped two guava populations (Race 1) and two cotton populations (Race cotton), with 95 % and 100 % bootstrap support, respectively. The two pepper populations clustered with two groups, and the sweet potato (race 1) population was the most divergent and clustered separately. Mitochondrial DNA (COII) study grouped the two cotton populations in race 2 from Brazil. Despite RAPD/AFLP and COII analysis, genetic traits that are linked to host races remain elusive. Ribosomal DNA (ITS, D2-D3), and the HSP90 gene revealed no interactions related to host races. This study emphasizes the importance of understanding the races and intraspecific variability in managing the impact of M. enterolobii through genetic resistance and crop rotation strategies.

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影响巴西作物的两种肠圆线虫的遗传多样性及主种试验新品种评价

本研究调查了巴西肠曲线虫（Meloidogyne enterolobii）种群的寄主种和遗传多样性，肠曲线虫是一种重要的线虫物种，其寄主范围广泛，对热带农业构成重大威胁。北卡罗莱纳差异寄主试验（NCDHT）显示了5个巴西人群中不同的致病谱，支持肠杆菌的亚分类分为两个生理小种：小种1（对番茄、烟草、西瓜和辣椒有阳性反应，但对棉花和花生没有）和小种2（对棉花也有阳性反应，但对花生没有）。由于巴西没有标准的NCDHT品种，我们评估了当地现有品种作为潜在替代品的兼容性。推荐的鉴别诊断品种为番茄‘Santa Clara’、辣椒‘Magali R’、西瓜‘Crimson sweet’、花生‘IAC Tatu’、烟草‘NC4’和棉花‘FM966’。具有相似遗传背景的其他品种也可能是合适的。利用RAPD和AFLP引物评估遗传变异。肠肠双歧杆菌种群间的变异性较低。串联邻居连接分析将两个番石榴种群（Race 1）和两个棉花种群（Race cotton）分组，分别具有95%和100%的bootstrap支持。2个辣椒居群聚为2个群体，甘薯（1种）居群分化最大，聚为单独居群。线粒体DNA （COII）研究将来自巴西的2种棉花种群进行了分组。尽管进行了RAPD/AFLP和COII分析，但与宿主种族相关的遗传性状仍然难以捉摸。核糖体DNA （ITS, D2-D3）和HSP90基因未发现与宿主种族相关的相互作用。本研究强调了通过遗传抗性和作物轮作策略，了解种内变异性和种内变异性对管理肠弧菌影响的重要性。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.