Comparative transcriptome analysis reveals defense mechanisms of Bacillus velezensis ZY1 against bacterial fruit blotch in watermelon

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-09-18 DOI:10.1016/j.pmpp.2025.102956

Haoyu Wei , Tingchang Zhao , Yushanjiang Maimaiti , Tielin Wang , Sheng Han , Wei Guan , Yuwen Yang

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引用次数: 0

Abstract

Watermelon (Citrullus lanatus) is a globally important horticultural crop, yet its production is severely constrained by bacterial fruit blotch (BFB), a destructive disease caused by Acidovorax citrulli. This study investigates the biocontrol potential of Bacillus velezensis ZY1 in promoting watermelon growth and suppressing BFB. The results demonstrate that B. velezensis ZY1 effectively inhibits A. citrulli proliferation while enhancing plant immunity by upregulating defense-related genes and increasing the activities of key defense enzymes, including superoxide dismutase, polyphenol oxidase, catalase, peroxidase, β-1,3-glucanase, chitinase, and lipoxygenase. To elucidate the molecular mechanisms underlying B. velezensis ZY1-mediated biocontrol, it was conducted a comparative transcriptome analysis of watermelon true leaves treated with B. velezensis ZY1 versus a sterile water control. Transcriptome sequencing identified 1688 up-regulated and 479 down-regulated genes in plants exposed to B. velezensis ZY1. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses revealed significant activation of transcription factors and disease resistance genes, which were further validated by quantitative retrotranscripted PCR. Differential expression analysis indicated that B. velezensis ZY1 modulates the mitogen-activated protein kinase (MAPK) and plant hormone signaling pathways, enhancing plant resistance to pathogens. This study expands transcriptomic resources and provides a molecular framework for understanding B. velezensis ZY1-induced systemic resistance against BFB, offering an effective strategy for controlling A. citrulli.

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比较转录组分析揭示了velezensis ZY1对西瓜细菌性果斑病的防御机制

西瓜（Citrullus lanatus）是一种全球重要的园艺作物，但其生产受到瓜酸霉（Acidovorax citrulli）引起的细菌性水果斑病（BFB）的严重制约。本研究探讨了velezensis ZY1在促进西瓜生长和抑制BFB方面的生物防治潜力。结果表明，B. velezensis ZY1通过上调防御相关基因，提高超氧化物歧化酶、多酚氧化酶、过氧化氢酶、过氧化物酶、β-1,3葡聚糖酶、几丁质酶和脂氧合酶等关键防御酶的活性，有效抑制瓜蒌增殖，增强植物免疫力。为了阐明白弧菌ZY1介导的生物防治的分子机制，本研究对西瓜真叶进行了转录组对比分析，分析了白弧菌ZY1处理与无菌水对照的效果。转录组测序结果显示，暴露于白叶草ZY1的植物中有1688个基因表达上调，479个基因表达下调。基因本体和京都基因与基因组百科全书路径富集分析显示转录因子和抗病基因显著激活，并通过定量反转录PCR进一步验证。差异表达分析表明，白螺旋体ZY1可调节丝裂原活化蛋白激酶（MAPK）和植物激素信号通路，增强植物对病原菌的抗性。本研究扩展了转录组学资源，为了解瓜蚜zy1诱导的对BFB的系统性抗性提供了分子框架，为瓜蚜控制提供了有效策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.