Detection and molecular characterization of phytoplasma associated with cherry trees in Himachal Pradesh (India)

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-09-15 DOI:10.1016/j.cropro.2025.107405

Aman Chauhan , Anil Handa , Ajay Brakta , Pankaj Kumar , Bhupesh Gupta , Rakesh Daroch , Abhinav Thakur

引用次数: 0

Abstract

During the surveys conducted in year 2023–2024 in cherry orchards of Himachal Pradesh, India, several cherry trees were found to be severely infected by a phloem residing pathogen showing the symptoms like little leaf, leaf rolling, leaf reddening, yellowing, tree decline and dieback. Fluorescence based microscopy and PCR assay of the infected plant samples showed the presence of phytoplasma in cherry plants. Presence of bright fluorescent bodies in phloem tissues and sequence analysis of 1239 bp 16S-23S rDNA amplified fragment by nested-PCR confirmed the association of Candidatus phytoplasma ziziphi belonging to 16SrV-B sub-group with declining cherry trees. The present research is the first report of infection by Ca. phytoplasma ziziphi in cherry trees in India.

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印度喜马偕尔邦樱桃树相关植物原体的检测及分子特性研究

在2023-2024年对印度喜马偕尔邦的樱桃园进行的调查中，发现几棵樱桃树被韧皮部寄生病原体严重感染，表现出小叶、卷叶、叶变红、变黄、树衰和枯死等症状。荧光显微镜和PCR检测结果显示，樱桃植株中存在植原体。在韧皮部组织中发现了明亮的荧光体，并通过巢式pcr对1239bp的16S-23S rDNA扩增片段进行了序列分析，证实了ziziphi候选菌属16SrV-B亚群与樱桃树衰退的相关性。本研究是首次报道印度樱桃树感染紫僵菌原体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.