Hydrogen peroxide–sodium carbonate pretreatment improves lignocellulose deconstruction and enzymatic saccharification of corn stover

IF 5.8 2区生物学 Q1 AGRICULTURAL ENGINEERING

Biomass & Bioenergy Pub Date : 2025-09-18 DOI:10.1016/j.biombioe.2025.108402

Zejin Li , Zuhua Zhao , Lu Zhang , Linna Suo

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Abstract

Lignocellulose recalcitrance remains a major barrier to efficient biomass conversion. In this study, corn stover (CS) was pretreated with low concentrations of hydrogen peroxide (1–2 % w/v) in combination with H₂SO₄, NaOH, or Na₂CO₃. Among these, the Na₂CO₃–H₂O₂ system achieved the most effective balance between wall polymer co-extraction and lignin removal, retaining 96.1 % glucan and 85.7 % xylan while eliminating 79.5 % lignin. Structural characterization confirmed that H₂O₂-induced oxidation substantially enhanced porosity and delignification. Orthogonal optimization (L₉ (3⁴)) identified the optimal conditions (120 °C, 12:1 liquid–solid ratio, 80 min), yielding 76.5 % total sugar (422.9 mg/g substrate), a 26.9 % improvement over unoptimized conditions. This mild and environmentally benign pretreatment markedly promoted enzymatic hydrolysis efficiency and demonstrates strong potential for low-cost biofuel production from agricultural residues.

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过氧化氢-碳酸钠预处理改善了木质纤维素的分解和玉米秸秆的酶解糖化

木质纤维素的顽固性仍然是有效生物质转化的主要障碍。在这项研究中，用低浓度的双氧水（1 - 2% w/v）与H2SO4、NaOH或Na2CO3联合预处理玉米秸秆（CS）。其中，Na2CO3-H2O2体系在壁聚合物共萃取和木质素去除之间达到了最有效的平衡，保留了96.1%的葡聚糖和85.7%的木聚糖，同时去除了79.5%的木质素。结构表征证实h2o2诱导氧化大大增强了孔隙度和脱木质素。正交优化（L9(34)）确定了最佳条件（120°C， 12:1液固比，80 min），总糖收率为76.5% （422.9 mg/g底物），比未优化条件提高26.9%。这种温和且环保的预处理显著提高了酶水解效率，并显示出从农业残留物中生产低成本生物燃料的巨大潜力。

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来源期刊

Biomass & Bioenergy 工程技术-能源与燃料

CiteScore

11.50

自引率

3.30%

发文量

258

审稿时长

60 days

期刊介绍： Biomass & Bioenergy is an international journal publishing original research papers and short communications, review articles and case studies on biological resources, chemical and biological processes, and biomass products for new renewable sources of energy and materials. The scope of the journal extends to the environmental, management and economic aspects of biomass and bioenergy. Key areas covered by the journal: • Biomass: sources, energy crop production processes, genetic improvements, composition. Please note that research on these biomass subjects must be linked directly to bioenergy generation. • Biological Residues: residues/rests from agricultural production, forestry and plantations (palm, sugar etc), processing industries, and municipal sources (MSW). Papers on the use of biomass residues through innovative processes/technological novelty and/or consideration of feedstock/system sustainability (or unsustainability) are welcomed. However waste treatment processes and pollution control or mitigation which are only tangentially related to bioenergy are not in the scope of the journal, as they are more suited to publications in the environmental arena. Papers that describe conventional waste streams (ie well described in existing literature) that do not empirically address ''new'' added value from the process are not suitable for submission to the journal. • Bioenergy Processes: fermentations, thermochemical conversions, liquid and gaseous fuels, and petrochemical substitutes • Bioenergy Utilization: direct combustion, gasification, electricity production, chemical processes, and by-product remediation • Biomass and the Environment: carbon cycle, the net energy efficiency of bioenergy systems, assessment of sustainability, and biodiversity issues.