Bulk segregated analysis, transcriptome, and functional analyses reveal the involvement of VcKCS19 and VcCER2 in regulating blueberry fruit wax accumulation

IF 6.2 1区 农林科学 Q1 HORTICULTURE
Liwei Chu, Xueting Wang, Wanting Li, Siyu Zuo, Yawen Kang, Wenji Zhang, Hexin Wang, Rongli Gai, Guohui Xu
{"title":"Bulk segregated analysis, transcriptome, and functional analyses reveal the involvement of VcKCS19 and VcCER2 in regulating blueberry fruit wax accumulation","authors":"Liwei Chu, Xueting Wang, Wanting Li, Siyu Zuo, Yawen Kang, Wenji Zhang, Hexin Wang, Rongli Gai, Guohui Xu","doi":"10.1016/j.hpj.2025.07.006","DOIUrl":null,"url":null,"abstract":"The blueberry (<ce:italic>Vaccinium</ce:italic> L.) fruit is known for its light-blue appearance, attributed to its blue-black skin covered with a white waxy layer. This layer is crucial for the growth and storage of blueberry fruits. This study established an F<ce:inf loc=\"post\">1</ce:inf> mapping population of 166 plants derived from ‘Chandler’ (wax-rich) and ‘Black Pearl’ (wax-poor). Bulk segregated analysis sequencing was used to identify the candidate region associated with cuticular wax, which was finally narrowed to a 1.85 Mb segment on chromosome 11, including ten candidate genes. Further transcriptome analyses and qRT-PCR revealed that the relative expression levels of five candidate genes (<ce:italic>VcMYB36</ce:italic>, <ce:italic>VcDXS</ce:italic>, <ce:italic>VcCAMS1</ce:italic>, <ce:italic>VcCER2,</ce:italic> and <ce:italic>VcKCS19</ce:italic>) were different between ‘Chandler’ and ‘Black Pearl’. Combined with gene function annotation, we hypothesized that <ce:italic>VcKCS19</ce:italic> and <ce:italic>VcCER2</ce:italic> are key candidate genes for wax synthesis in blueberry, potentially accounting for the variations in wax content between ‘Chandler’ and ‘Black Pearl’. Transient overexpression and virus-induced gene silencing in blueberry fruit revealed that <ce:italic>VcCER2</ce:italic> and <ce:italic>VcKCS19</ce:italic> positively regulate wax accumulation in blueberry fruits. This study provided key insights into the molecular basis of wax synthesis in blueberry fruits, which provides new avenues for enhancing wax quality in genetic breeding programs.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"39 1","pages":""},"PeriodicalIF":6.2000,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2025.07.006","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0

Abstract

The blueberry (Vaccinium L.) fruit is known for its light-blue appearance, attributed to its blue-black skin covered with a white waxy layer. This layer is crucial for the growth and storage of blueberry fruits. This study established an F1 mapping population of 166 plants derived from ‘Chandler’ (wax-rich) and ‘Black Pearl’ (wax-poor). Bulk segregated analysis sequencing was used to identify the candidate region associated with cuticular wax, which was finally narrowed to a 1.85 Mb segment on chromosome 11, including ten candidate genes. Further transcriptome analyses and qRT-PCR revealed that the relative expression levels of five candidate genes (VcMYB36, VcDXS, VcCAMS1, VcCER2, and VcKCS19) were different between ‘Chandler’ and ‘Black Pearl’. Combined with gene function annotation, we hypothesized that VcKCS19 and VcCER2 are key candidate genes for wax synthesis in blueberry, potentially accounting for the variations in wax content between ‘Chandler’ and ‘Black Pearl’. Transient overexpression and virus-induced gene silencing in blueberry fruit revealed that VcCER2 and VcKCS19 positively regulate wax accumulation in blueberry fruits. This study provided key insights into the molecular basis of wax synthesis in blueberry fruits, which provides new avenues for enhancing wax quality in genetic breeding programs.
大量分离分析、转录组和功能分析显示VcKCS19和VcCER2参与蓝莓果蜡积累的调控
蓝莓(Vaccinium L.)果实以其浅蓝色的外观而闻名,这要归功于它蓝黑色的皮肤上覆盖着一层白色的蜡质层。这一层对蓝莓果实的生长和储存至关重要。本研究建立了来自‘Chandler’(富含蜡质)和‘Black Pearl’(缺乏蜡质)的166株植物的F1图谱群体。利用Bulk segregated analysis sequencing对候选区域进行筛选,最终将候选区域缩小到11号染色体上1.85 Mb的片段,包含10个候选基因。进一步的转录组分析和qRT-PCR显示,“钱德勒”和“黑珍珠”的5个候选基因(VcMYB36、VcDXS、VcCAMS1、VcCER2和VcKCS19)的相对表达水平存在差异。结合基因功能注释,我们假设VcKCS19和VcCER2是蓝莓蜡合成的关键候选基因,可能解释了“钱德勒”和“黑珍珠”之间蜡含量的差异。VcCER2和VcKCS19在蓝莓果实中的瞬时过表达和病毒诱导的基因沉默表明,VcCER2和VcKCS19正调控蓝莓果实的蜡积累。该研究为蓝莓果实蜡合成的分子基础提供了重要的见解,为遗传育种中提高蜡质提供了新的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信