Metal-free microRNA-320b electrochemical sensor based on blue light-driven ring-opening metathesis polymerization signal amplification

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-09-20 DOI:10.1016/j.snb.2025.138809

Shijin Zhou , Qinyuan Xu , Zhidan Tian , Jinming Kong , Xueji Zhang

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Abstract

Developing efficient strategies for the sensitive and accurate detection of esophageal squamous cell carcinoma (ESCC) biomarkers is critical for improving early diagnosis and preventive interventions. An electrochemical sensor incorporating a photo-redox mediated ring-opening metathesis polymerization (photo-ROMP) strategy was designed to achieve sensitive and selective detection of microRNA-320b (miRNA-320b), a key biomarker for ESCC. Two DNA strands served as capture probes to form a sandwich structure with miRNA-320b, and the initiator 2-(vinyloxy)ethanol (VEOH) was chemically attached to the DNA terminus. Upon blue LED irradiation, the photosensitizer 2,4,6-Tris(4-methoxyphenyl)pyridinium tetrafluoroborate becomes excited, oxidizes VEOH, generates active radical cations, and initiates the polymerization reaction, thereby amplifying the detection signal. The VEOH-triggered photo-ROMP strategy provides the advantages of metal-free operation, improved sensitivity, and simplified handling. Consistently, the method achieves a remarkably low detection limit of 1.1 fM, demonstrating its high sensitivity. In addition, the sensor exhibits good selectivity, stability and anti-interference ability, and still maintain excellent performance in complex actual samples, showing its application potential in early screening of ESCC.

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基于蓝光驱动开环复分解聚合信号放大的无金属微rna -320b电化学传感器

制定有效的策略来敏感和准确地检测食管鳞状细胞癌（ESCC）的生物标志物对于改善早期诊断和预防干预至关重要。采用光氧化还原介导的开环复分解聚合（photoromp）策略设计了一种电化学传感器，以实现对ESCC关键生物标志物microRNA-320b （miRNA-320b）的敏感和选择性检测。两条DNA链作为捕获探针与miRNA-320b形成三明治结构，引发剂2-（乙烯氧基）乙醇（VEOH）化学附着在DNA末端。在蓝光LED照射下，光敏剂2,4,6-三（4-甲氧基苯基）四氟硼酸吡啶被激发，氧化VEOH，生成活性自由基阳离子，引发聚合反应，从而放大检测信号。veoh触发的photo-ROMP策略具有无金属操作、提高灵敏度和简化操作的优点。同时，该方法的检测限非常低，仅为1.1 fM，具有很高的灵敏度。此外，该传感器具有良好的选择性、稳定性和抗干扰能力，在复杂的实际样品中仍保持优异的性能，显示了其在ESCC早期筛选中的应用潜力。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.