A novel strategy for construction of a ratio dual signal electrochemical sensing platform to detect homocysteine

IF 5.6 3区材料科学 Q1 ELECTROCHEMISTRY

Electrochimica Acta Pub Date : 2025-09-20 DOI:10.1016/j.electacta.2025.147432

Xiaoli Zhang, Yan Zhang, Hongna Ma, Yali Yang, Xiuhui Liu

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引用次数: 0

Abstract

Reliable and selective sensing of homocysteine (Hcy) is essential for early screening and prevention of chronic diseases in elderly populations. In this work, we proposed a new " on-off " signal ratio sensing strategy. Specifically, homocysteine (Hcy) and catechol (Cat) were chosen as dual-signal probes, and the ratio sensing perform of Hcy was constructed by monitoring the dynamic changes of I_4-SH-Cat/I_Cat and I_Hcy/I_Cat. The linear response range covered 5.76 × 10^-⁹ ∼ 1.23 × 10^-⁴ M, with a low detection limit of 1.92 nM. Furthermore, the electrochemical reaction mechanism was explored thoroughly based on 1,4-Michael addition reaction between Hcy and Cat. Importantly, the proposed ratio electrochemical method can selectively identify Hcy in the presence of cysteine (Cys). Therefore, the study provides an effective method for realizing fast, reliable and sensitive determination of Hcy, and exhibits significant application value in biomedical analysis and clinical diagnosis for early screening of Hcy-related diseases.

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构建比值双信号电化学传感平台检测同型半胱氨酸的新策略

可靠和选择性地检测同型半胱氨酸（Hcy）对老年人群的早期筛查和预防慢性疾病至关重要。在这项工作中，我们提出了一种新的“开关”信号比检测策略。其中，选择同型半胱氨酸（Hcy）和儿茶酚（Cat）作为双信号探针，通过监测I4-SH-Cat/ICat和IHcy/ICat的动态变化，构建Hcy的比值传感性能。线性响应范围为5.76 × 10-⁹~ 1.23 × 10-⁴M，低检出限为1.92 nM。进一步探讨了Hcy与Cat之间的1,4- michael加成反应的电化学机理。重要的是，所提出的比例电化学方法可以选择性地识别存在半胱氨酸（Cys）的Hcy。因此，本研究为实现Hcy快速、可靠、灵敏的检测提供了一种有效的方法，在早期筛查Hcy相关疾病的生物医学分析和临床诊断中具有重要的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Electrochimica Acta 工程技术-电化学

CiteScore

11.30

自引率

6.10%

发文量

1634

审稿时长

41 days

期刊介绍： Electrochimica Acta is an international journal. It is intended for the publication of both original work and reviews in the field of electrochemistry. Electrochemistry should be interpreted to mean any of the research fields covered by the Divisions of the International Society of Electrochemistry listed below, as well as emerging scientific domains covered by ISE New Topics Committee.