Ultrasensitive Electrochemical Detection of Amyloid-β Peptide Using a Homochiral Metal-Organic Framework Binding to the l-Diphenylalanine Targeting Site.
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引用次数: 0
Abstract
The high sensitivity and accuracy of amyloid-β peptide (Aβ) detection could provide strong support for early diagnosis, monitoring of disease progression, and effective treatment of Alzheimer's disease (AD). Improving the specificity and affinity of sensing materials for Aβ is key to detecting Aβ. Herein, an electrochemical sensor based on an unmodified homochiral MOF, Zn-BPIleBp, was developed for the first time for the detection of Aβ1-40, with an ultralow detection limit of 1.7 pM (7.36 pg/mL). The Zn-BPIleBp sensor also displays high-efficiency enantioselectivity and ultrasensitivity in identifying diphenylalanine (PhePhe) corresponding to the core recognition motif of Aβ1-40, with a high peak current ratio (IL/ID) of 2.78, large potential difference (EL - ED) of 140 mV, and ultralow detection limit of 34 fM for l-PhePhe. The sensor has been successfully applied for the ultrasensitive quantification of l-PhePhe and Aβ1-40 in racemic mixtures, artificial cerebrospinal fluid (aCSF) and fetal bovine serum (FBS). According to structural and spectral analysis, the high sensitivity and affinity of homochiral MOF toward Aβ1-40, without the involvement of any biomolecular modifications such as antibodies or aptamers, stem from the structural, hydrophobic, and chiral matching between the framework and Aβ1-40. This sensor proffers a fast, highly stable, reproducible, ultrasensitive, and accurate detection method for Aβ1-40, demonstrating great potential in the clinical application of AD. This work also opens up new perspectives for designing sensing platforms using MOFs as sensing materials and expanding their functionality and applications to the fields of biological and medical analysis.
期刊介绍:
ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.