Monitoring hepatic cysteine dynamics in obesity models with sulfur-substituted hemicyanine-based nanoprobes

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-09-17 DOI:10.1016/j.saa.2025.126965

Jing-Jing Qiao , Lu Fan , Jie-Li Zhou , Kai Wang

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引用次数: 0

Abstract

Cysteine (Cys) plays pivotal roles in redox homeostasis and metabolic regulation, with its dysregulation implicated in obesity-related hepatic disorders. Here, we developed a sulfur-substituted hemicyanine-based nanoprobe (Cys-NP) for photoacoustic imaging of hepatic Cys dynamics. The Cys-responsive molecular probe (Cys-MP) was encapsulated in mPEG-DSPE nanoparticles to overcome aqueous solubility limitations, yielding monodisperse nanoparticles with an average diameter of 28.7 nm and excellent colloidal stability. Sulfur substitution endowed the probe enhanced photoacoustic signals compared to oxygenated analogs, while selective Cys recognition triggered a bathochromic shift and yielded a detection limit of 0.13 μM. In high-fat-diet-induced obese mice, Cys-NP revealed significantly elevated hepatic Cys levels correlating with histopathological steatosis and hypercholesterolemia. This work establishes a nanotechnology-enabled strategy for mapping redox metabolism in metabolic diseases, offering both mechanistic insights and diagnostic potential.

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用硫代半花青碱纳米探针监测肥胖模型中肝脏半胱氨酸动力学。

半胱氨酸（Cys）在氧化还原稳态和代谢调节中起关键作用，其失调与肥胖相关的肝脏疾病有关。在这里，我们开发了一种基于硫取代半花青碱的纳米探针（Cys- np），用于肝脏Cys动力学的光声成像。cys -响应分子探针（Cys-MP）被包裹在mPEG-DSPE纳米颗粒中，以克服水溶性限制，得到平均直径为28.7 nm的单分散纳米颗粒，具有优异的胶体稳定性。与含氧类似物相比，硫取代赋予探针增强的光声信号，而选择性Cys识别引发了色移，检测限为0.13 μM。在高脂肪饮食诱导的肥胖小鼠中，Cys- np显示肝脏Cys水平显著升高，与组织病理学脂肪变性和高胆固醇血症相关。这项工作建立了一种纳米技术支持的策略，用于绘制代谢性疾病中的氧化还原代谢，提供了机制见解和诊断潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.