Highly sensitive and selective fluorescent detection of vitamin D3 in human serum using carbon quantum dots/nano-graphite hybrid sensor

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-09-12 DOI:10.1016/j.saa.2025.126936

Xinqiu Xu , Junbo Zhang , Qihui Wang , Yehao Yan , Ziheng Wang , Zhi Li , Yujia Wang , Yin Wei

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Abstract

This study explores the use of carbon quantum dots (CQDs), aptamers (Apt), and nanographite (NG) for fluorescent sensing of vitamin D. CQDs-Apt conjugates adsorb onto the surface of NG via π-π stacking interactions, inducing an internal filter effect (IFE) that quenches the intrinsic fluorescence of the CQDs-Apt complex. In the presence of vitamin D, this internal filter effect is disrupted, resulting in the recovery of fluorescence from the CQDs-Apt conjugates. The sensor exhibits a linear response range from 0 to 900 nM with a detection limit of 20.87 nM (calculated using the 3σ method). Importantly, this sensor has been successfully applied to detect vitamin D in blood samples. Furthermore, the aptamer sensors based on carbon quantum dots can effectively distinguish the interfering substances in serum. This technology offers advantages such as high sensitivity, selectivity, simplicity of operation, thus opening new avenues for the development of vitamin sensing platforms.

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碳量子点/纳米石墨混合传感器对人血清维生素D3的高灵敏度和选择性荧光检测。

本研究探索了利用碳量子点（CQDs）、适体（Apt）和纳米石墨（NG）对维生素d进行荧光传感。CQDs-Apt缀合物通过π-π堆叠相互作用吸附在NG表面，诱导内部过滤效应（IFE），猝灭CQDs-Apt复合物的固有荧光。在维生素D的存在下，这种内部过滤效应被破坏，导致CQDs-Apt偶联物的荧光恢复。传感器的线性响应范围为0 ~ 900 nM，检测限为20.87 nM（采用3σ法计算）。重要的是，这种传感器已经成功地应用于检测血液样本中的维生素D。此外，基于碳量子点的适体传感器能够有效区分血清中的干扰物质。该技术具有灵敏度高、选择性好、操作简单等优点，为维生素传感平台的开发开辟了新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.