Long noncoding RNA DQ786243 and miRNA146a as potential salivary biomarkers for selected oral potentially malignant disorders: a diagnostic accuracy study.

IF 1.9 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2025-08-27 DOI:10.1016/j.oooo.2025.08.013

Salsabeel Afifi, Sally Ibrahim, Ali Aw Kotb, Sahar ElRefai, Layla Hafed, Olfat Shaker, Safaa Baz, Rania Shalaby

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引用次数: 0

Abstract

Objective: Assess the diagnostic accuracy of salivary long noncoding RNA (lncRNA) DQ786243 and miRNA-146a as potential biomarkers for differentiating oral lichen planus (OLP) and oral leukoplakia (OL), both belonging to oral potentially malignant disorders (OPMDs), from healthy controls.

Study design: Forty-five subjects were enrolled in a consecutive manner and allocated into 3 groups. Group I: 20 participants suffering from OLP. Group II: 15 patients with OL. Group III: 10 healthy controls. Expression of DQ786243 and miRNA146a in saliva was measured using quantitative real-time polymerase chain reaction. Reference standard was histopathological examination of OLP and OL.

Results: The greatest elevation of DQ8786243 and miRNA146a fold change (FC) occurred in OLP patients, especially erosive type, followed by OL group, whereas it was the lowest in healthy controls. Sensitivity and specificity of salivary DQ8786243 were 97.14% and 100%, respectively, at a cut point of 1.8 FC, delineating the difference between disease and control. Concerning miRNA146a, at a cut point of 1.4 FC, sensitivity and specificity were 94.29% and 100%, respectively.

Conclusions: LncRNA DQ786243 and miRNA146a could be promising salivary biomarkers in the detection of OPMDs, especially OLP and OL. Salivary expression of both markers was the highest in erosive OLP.

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长链非编码RNA DQ786243和miRNA146a作为口腔潜在恶性疾病的潜在唾液生物标志物：一项诊断准确性研究

目的：评估唾液长链非编码RNA (lncRNA) DQ786243和miRNA-146a作为区分口腔扁平苔藓（OLP）和口腔白斑（OL）与健康对照的潜在生物标志物的诊断准确性，这两种疾病都属于口腔潜在恶性疾病（OPMDs）。研究设计：45名受试者连续入组，分为3组。第一组：OLP患者20人。第二组：OL患者15例。第三组：10名健康对照。采用实时定量聚合酶链反应检测唾液中DQ786243和miRNA146a的表达。参照标准为OLP和OL的组织病理学检查。结果：DQ8786243和miRNA146a fold change （FC）在OLP患者中以糜烂型最高，OL组次之，健康对照组最低。唾液DQ8786243的敏感性和特异性分别为97.14%和100%，切点为1.8 FC，描绘了疾病与对照组的差异。miRNA146a在1.4 FC切割点时，敏感性为94.29%，特异性为100%。结论：LncRNA DQ786243和miRNA146a可能是检测opmd，特别是OLP和OL的有希望的唾液生物标志物。在糜烂性OLP中，两种标记物的唾液表达量最高。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.