Wenlun Wang, Huang Yan, Li Wenbin, Yu Chaohang, Wu Shengcai, Cai Xioqing, Zhou Nanqing, Yuan Qing, Hu Qianmin, Zhang Feng, Zhu Lingyun
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引用次数: 0
Abstract
Cell proliferation plays a crucial role in muscle atrophy-regeneration. However, the functional roles of Long non-coding RNA X-inactive specific transcript (lncRNA Xist) in C2C12 cell proliferation remain poorly characterized. We identified lncRNA Xist as a regulator of cell proliferation kinetics by acting as a competing endogenous RNA (ceRNA) in C2C12 cells. Downregulation or overexpression of lncRNA Xist correlated with enhanced or impaired cell proliferation, respectively. Mechanistically, lncRNA Xist sponges miR-486-5p to regulate serine/threonine kinase 4 (Stk4) expression, thereby modulating C2C12 cell proliferation in vitro. The lncRNA Xist/miR-486-5p/Stk4 ceRNA network plays an essential role in C2C12 proliferation, suggesting lncRNA Xist as a potential therapeutic target for muscle atrophy.
期刊介绍：
The Journal of Muscle Research and Cell Motility has as its main aim the publication of original research which bears on either the excitation and contraction of muscle, the analysis of any one of the processes involved therein, the processes underlying contractility and motility of animal and plant cells, the toxicology and pharmacology related to contractility, or the formation, dynamics and turnover of contractile structures in muscle and non-muscle cells. Studies describing the impact of pathogenic mutations in genes encoding components of contractile structures in humans or animals are welcome, provided they offer mechanistic insight into the disease process or the underlying gene function. The policy of the Journal is to encourage any form of novel practical study whatever its specialist interest, as long as it falls within this broad field. Theoretical essays are welcome provided that they are concise and suggest practical ways in which they may be tested. Manuscripts reporting new mutations in known disease genes without validation and mechanistic insight will not be considered. It is the policy of the journal that cells lines, hybridomas and DNA clones should be made available by the developers to any qualified investigator. Submission of a manuscript for publication constitutes an agreement of the authors to abide by this principle.
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