{"title":"A Selective Fluorescent Probe with Pyridine-Imidazole Moiety for Detecting Glutathione in Pediatric Cellular Colorectal Injury.","authors":"Li-Mei Tian, Wen Yang, Jing-Yi Shi","doi":"10.1007/s10895-025-04569-4","DOIUrl":null,"url":null,"abstract":"<p><p>In this work, a selective fluorescent probe PIOr-GSH with pyridine-imidazole moiety was developed for detecting glutathione in pediatric cellular colorectal injury. Compared with the recent probes with the similar backbone, the modification of the hydroxyl position herein resulted in the enhancement of the signal intensity at 485 nm with the excitation of 310 nm. On the optical capabilities, the detecting system with PIOr-GSH exhibited the advantages including applicable linear range, high sensitivity, high steadiness in various working conditions (recognition time, pH, and incubation temperature). In particular, PIOr-GSH achieved the convenient and specific recognition towards GSH from the competing species including Cys/Hcy. As a challenging trial, PIOr-GSH achieved imaging the pediatric cellular colorectal injury status and the treatment efficacy by monitoring the intracellular GSH level. This work was meaningful for the rapid diagnosis and urgent therapeutics in the pediatric emergency.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04569-4","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
In this work, a selective fluorescent probe PIOr-GSH with pyridine-imidazole moiety was developed for detecting glutathione in pediatric cellular colorectal injury. Compared with the recent probes with the similar backbone, the modification of the hydroxyl position herein resulted in the enhancement of the signal intensity at 485 nm with the excitation of 310 nm. On the optical capabilities, the detecting system with PIOr-GSH exhibited the advantages including applicable linear range, high sensitivity, high steadiness in various working conditions (recognition time, pH, and incubation temperature). In particular, PIOr-GSH achieved the convenient and specific recognition towards GSH from the competing species including Cys/Hcy. As a challenging trial, PIOr-GSH achieved imaging the pediatric cellular colorectal injury status and the treatment efficacy by monitoring the intracellular GSH level. This work was meaningful for the rapid diagnosis and urgent therapeutics in the pediatric emergency.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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