Potential human health risk of carbapenem-non-susceptible Pseudomonas aeruginosa from companion animals.

IF 3.6 2区 医学 Q1 INFECTIOUS DISEASES
Jirachaya Toyting-Hiraishi, Toyotaka Sato, Mana Tohyama, Taro Fujino, Kaho Okada, Kazuyoshi Sasaoka, Nozomu Yokoyama, Kana Torii, Akio Suzuki, Yuzo Tsuyuki, Kensuke Nakamura, Mitsuyoshi Takiguchi, Motohiro Horiuchi
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Abstract

Background and objectives: The close bond between companion animals and humans may accelerate the spread of antimicrobial-resistant bacteria. Pseudomonas aeruginosa, an opportunistic pathogen in both, poses a public health threat due to antimicrobial resistance (AMR) and diverse virulence factors. However, One Health-based comparison remains limited. This study investigated the current AMR status and molecular characteristics of P. aeruginosa in companion animals in Japan to assess potential human health risks.

Methods: We examined 197 P. aeruginosa clinical isolates from companion animals [dogs (n = 99) and cats (n = 98)] across Japan in 2024. Antimicrobial susceptibility to human clinical antibiotics was evaluated. In carbapenem-non-susceptible isolates, multilocus sequence typing and detection of resistance genes and virulence factors were performed.

Results: Ciprofloxacin (20.3%) and piperacillin (10.7%) showed the highest resistance rates, with 5.6% of isolates being multidrug-resistant. Carbapenem resistance rates were 6.1% for imipenem and 1.0% for meropenem. Thirty-five isolates (17.8%) exhibited carbapenem non-susceptibility but remained susceptible to cefepime, ciprofloxacin, or amikacin. Of 27 identified sequence types, 20 (77.1% of carbapenem-non-susceptible isolates) were known in humans, including two high-risk clones (ST233 and ST298; 8.6%) reported for the first time in Japanese companion animals. These isolates carried mutations in efflux pump-related genes and multiple virulence factors. One showed close genetic relatedness to a human isolate, suggesting possible interspecies transmission.

Conclusions: Our findings highlight the potential cross-species transmission risk of antimicrobial-resistant P. aeruginosa. Identification of shared high-risk clones with multiple virulence factors emphasizes the need for continuous vigilance and actions within the One Health framework.

来自伴侣动物的碳青霉烯非敏感铜绿假单胞菌的潜在人类健康风险。
背景与目的:伴侣动物与人类之间的密切联系可能会加速耐药细菌的传播。铜绿假单胞菌是两者的机会致病菌,由于抗菌素耐药性和多种毒力因素,对公共卫生构成威胁。然而,一种基于健康的比较仍然有限。本研究调查了日本伴侣动物中铜绿假单胞菌的AMR现状和分子特征,以评估潜在的人类健康风险。方法:对2024年从日本各地的伴侣动物[狗(n = 99)和猫(n = 98)]中分离出的197株铜绿假单胞菌进行临床检测。评估人类临床抗生素的药敏。对碳青霉烯不敏感菌株进行多位点序列分型、耐药基因和毒力因子检测。结果:环丙沙星(20.3%)和哌拉西林(10.7%)的耐药率最高,其中多重耐药占5.6%;亚胺培南的碳青霉烯耐药率为6.1%,美罗培南为1.0%。35株(17.8%)对碳青霉烯不敏感,但对头孢吡肟、环丙沙星或阿米卡星敏感。在鉴定的27种序列类型中,人类已知20种(77.1%的碳青霉烯类非敏感分离株),其中包括首次在日本伴侣动物中报告的两个高风险克隆(ST233和ST298; 8.6%)。这些分离物携带外排泵相关基因和多种毒力因子的突变。其中一种显示出与人类分离物的密切遗传关系,表明可能存在种间传播。结论:我们的发现强调了耐药铜绿假单胞菌潜在的跨物种传播风险。识别具有多种毒力因素的共同高风险克隆,强调需要在“同一个健康”框架内持续保持警惕和采取行动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
9.20
自引率
5.80%
发文量
423
审稿时长
2-4 weeks
期刊介绍: The Journal publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents. The Journal publishes primarily in human medicine, and articles in veterinary medicine likely to have an impact on global health.
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