Effect of re-biopsy and re-vitrification on clinical outcomes in preimplantation genetic testing for aneuploidy.

IF 2.7 3区医学 Q2 GENETICS & HEREDITY

Journal of Assisted Reproduction and Genetics Pub Date : 2025-09-20 DOI:10.1007/s10815-025-03675-3

Huijiao Wu, Hui Wang, Yunhao Liang, Yu Deng, Yu Jiang, Li Yang, Shuai Liu, Zhiheng Chen

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引用次数: 0

Abstract

Purpose: To investigate whether re-biopsy and re-vitrification affect embryo developmental potential and clinical outcomes.

Methods: Embryos that underwent preimplantation genetic testing for aneuploidy (PGT-A) and single embryo transfer between December 2017 and April 2024 were studied. All embryos were categorized into three groups: fresh biopsy and vitrification (BV group; n = 346); thawed, biopsied, and subsequently vitrified (VBV group; n = 12); fresh biopsied, vitrified, further thawed and biopsied again due to inconclusive results from the previous biopsy (BVBV group; n = 19). The live birth rate and miscarriage rate will be evaluated after embryo transfer in each group.

Results: Live birth rate (LBR) was not statistically significant among the BV group (50.00%), VBV group [41.67%; adjusted relative risks (aRR) 0.96; 95% confidence interval (95% CI): 0.81-1.14], and BVBV group (42.11%; aRR 0.97, 95% CI: 0.83-1.13). The miscarriage rate was not statistically significant: BV group (11.27%); VBV group (25%; aRR 1.09, 95% CI: 0.95-1.25), and BVBV group (5.26%; aRR 0.97, 95% CI: 0.91-1.02).

Conclusions: The differences in LBR and miscarriage rates among the groups were not statistically significant. However, the limited sample sizes of the VBV and BVBV groups cannot rule out the possibility of moderate differences. Some embryos can be successfully salvaged, leading to live births. To minimize inconclusive results, the laboratory should refine the procedural details.

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再次活检和再次玻璃化对非整倍体植入前基因检测临床结果的影响。

目的：探讨再次活检和再次玻璃化是否会影响胚胎发育潜能和临床结果。方法：对2017年12月至2024年4月间进行非整倍体植入前基因检测（PGT-A）和单胚胎移植的胚胎进行研究。所有胚胎分为三组：新鲜活检和玻璃化（BV组，n = 346）；解冻，活检，然后玻璃化（VBV组，n = 12）；由于先前活检结果不确定，新鲜活检、玻璃化、进一步解冻和再次活检（BVBV组；n = 19）。评估各组胚胎移植后的活产率和流产率。结果：BV组活产率（LBR）为50.00%，VBV组为41.67%；调整相对危险度（aRR） 0.96；95%可信区间（95% CI）： 0.81-1.14]， BVBV组（42.11%;aRR 0.97, 95% CI: 0.83-1.13）。流产率差异无统计学意义：BV组（11.27%）；VBV组（25%;aRR 1.09, 95% CI: 0.95-1.25）和BVBV组（5.26%;aRR 0.97, 95% CI: 0.91-1.02）。结论：两组间LBR、流产率差异无统计学意义。然而，有限的VBV组和BVBV组的样本量不能排除适度差异的可能性。一些胚胎可以成功抢救，导致活产。为了尽量减少不确定的结果，实验室应完善程序细节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Assisted Reproduction and Genetics 医学-妇产科学

CiteScore

5.70

自引率

9.70%

发文量

286

审稿时长

1 months

期刊介绍： The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species. The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.