Histopathological effects of heavy metal on bivalves: Review and perspectives.

Abstract

Objective: We sought to establish a current understanding of the histopathology of bivalves following exposure to heavy metal and to standardize the related terminology and microscopic diagnostic techniques, highlight limitations that hinder understanding, and discuss future research perspectives.

Methods: We conducted a literature review, looking for studies that implemented histopathological analyses to describe the damage that is caused by heavy metal bioaccumulation. For further description, we categorized lesions as regressive, defensive, or progressive.

Results: We analyzed 49 articles that employed histopathological techniques to describe the morphological changes that are associated with heavy metal bioaccumulation in bivalves. Since the 1980s, a growing interest in understanding how heavy metals affect bivalves has been evident, given the increasing number of published articles. Most studies were descriptive, although some contained experimental methods. The descriptive studies described damage to the digestive gland, gonads, gills, and kidneys. Defensive lesions were the most common response in the literature, with inflammation events reported in most cases. Regressive and progressive lesions were also documented, with necrosis (regressive change) and fibrosis (progressive change) being the most commonly reported, although not always correctly identified using microscopy.

Conclusions: Histopathological studies on the effects of heavy metals on bivalve health are abundant, and the resulting morphologic lesions have been described extensively. However, most studies on heavy metal accumulation in bivalves have not followed a microscopic approach, focusing instead on using bivalves solely as sentinel or bioindicator species. A histopathologic evaluation is a common and important facet of pathologic analyses; however, we identified inconsistencies among diagnoses and misleading use of pathological terms. Therefore, we highlight the need to establish a systematic and standardized characterization of lesions at the microscopical level and their subsequent categorization to reduce the ambiguity of pathological descriptions. Implementing other methodological approaches in combination with histopathological analysis will improve our understanding of how heavy metals affect the health of bivalves.