Effect of Heterorhabditis bacteriophora (HB EN01) and Steinernema rarum (PAM 25) nematodes on the control of cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

IF 2.2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-09-09 DOI:10.1016/j.vetpar.2025.110604

E.R. Souza, J.G.C. Orozco, F.C. Duarte, L.C. Torres, M.C. Mendes

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引用次数: 0

Abstract

The excessive use of acaricides in the management of the tick Rhipicephalus (Boophilus) microplus has favored the development of resistance in the parasites, in addition to causing environmental contamination. In this context, biological control using entomopathogenic nematodes appears as a promising alternative. This study evaluated the efficacy of two isolates, Heterorhabditis bacteriophora (HB EN01) and Steinernema rarum (PAM 25), in the in vitro control of R. (B.) microplus. Two experiments were conducted with different concentrations of nematodes. In the first, fifteen engorged females were exposed to suspensions containing 100, 200 and 300 infective juveniles (IJs) per female. The second experiment was carried out in soil substrate, with a concentration of 200 IJs per female. Three application systems were tested: S1 (females placed on the substrate before application), S2 (suspension applied before the introduction of females) and S3 (females placed in a voile bag on the substrate). For S. rarum (PAM 25), there was no significant difference in oviposition inhibition, but larval hatching was lower at concentrations of 200 and 300 IJs/female compared to 100 (p < 0.05). H. bacteriophora (HB EN01) showed a significant reduction in oviposition only between concentrations of 100 and 300 IJs/female (p < 0.05), with no variation in larval hatching or efficacy. On the soil substrate, both isolates showed efficacy above 80 %, except for S. rarum in the voile system (60 %). Treated females showed darkening and dehydration from the 4th day onwards. The control group remained healthy. The results indicate that EPNs are an effective alternative in the control of R. (B.) microplus.

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嗜杆菌异habditis bacteriophora （hben01）和罕见斯坦纳马（Steinernema rarum, PAM 25）线虫对牛蜱（Acari: Ixodidae）微蜱（Boophilus）的防治效果

除造成环境污染外，过量使用杀螨剂还会导致微头蜱产生抗药性。在这种情况下，利用昆虫病原线虫进行生物防治似乎是一种很有前途的选择。本研究评价了两种分离株，即嗜菌异芽杆菌（hben01）和稀少斯坦纳菌（PAM 25）在体外控制微弧菌的效果。用不同浓度的线虫进行了两项实验。在第一个实验中，15只肿胀的雌性暴露于每只雌性含有100、200和300只传染性少年（IJs）的悬浮液中。第二次试验在土壤基质中进行，浓度为200 IJs /雌。试验了三种施用系统：S1（施用前将雌虫置于基质上），S2（在引入雌虫之前施用悬浮剂）和S3（将雌虫置于遮光袋中置于基质上）。在不同浓度浓度下，不同浓度的小黑麦（PAM 25）的产卵抑制效果无显著差异，但不同浓度的小黑麦（PAM 25）在200和300 IJs/雌时的幼虫孵化率低于100 IJs/雌

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.