Comparative Efficacy of Stromal Vascular Fraction and Adipose-derived Stem Cells in UVB-induced Skin Damage in Nude Mice.

IF 3 2区 医学 Q1 SURGERY
Hui-Yi Hsiao, Chao-Yi Lai, Yung-Chun Chang, Shih-Han Huang, Jung-Ju Huang
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引用次数: 0

Abstract

Background: Chronic UVB exposure accelerates skin aging by inducing oxidative stress and inflammation, leading to dehydration, reduced collagen density, and impaired epidermal and dermal integrity. Stem cell-based therapies, including the use of the stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs), have emerged as promising approaches for skin regeneration.

Objectives: This study evaluated the therapeutic effects of SVF and ASCs on UVB-induced skin damage.

Methods: The mice were exposed to UVB irradiation (290-310 nm) for eight weeks to establish a photoaging model. Following UVB exposure, the SVF or ASCs were injected into the dorsal skin, and the skin samples were analyzed.

Results: UVB exposure significantly reduced skin hydration (-26%), decreased collagen density, and led to a decrease in the thickness of the epidermal and dermal layers. The SVF and ASC treatments improved skin hydration, enhanced extracellular matrix remodeling, and increased collagen density. Both treatments reduced oxidative stress by decreasing reactive oxygen species (ROS) levels, with SVF-treated samples exhibiting increased BMAL1 expression. SVF and ASCs also promoted angiogenesis and lymphangiogenesis. A reduction in macrophage infiltration and a shift in polarization from the proinflammatory M1 phenotype to the anti-inflammatory M2 phenotype were also observed in the SVF and ASC groups, with ASCs exhibiting stronger immunomodulatory effects. The SVF and ASCs each displayed distinct therapeutic advantages in skin tissue restoration.

Conclusions: These findings highlight the potential of the SVF and ASCs as therapeutic strategies for UVB-induced skin aging, warranting further clinical investigation.

基质血管组分和脂肪干细胞对uvb诱导的裸鼠皮肤损伤的比较疗效。
背景:慢性UVB暴露通过诱导氧化应激和炎症加速皮肤老化,导致脱水、胶原蛋白密度降低、表皮和真皮完整性受损。基于干细胞的治疗,包括使用基质血管部分(SVF)和脂肪来源干细胞(ASCs),已经成为皮肤再生的有希望的方法。目的:评价SVF和ASCs对uvb致皮肤损伤的治疗效果。方法:用UVB (290 ~ 310 nm)照射小鼠8周,建立光老化模型。在UVB照射后,将SVF或ASCs注射到背侧皮肤,并对皮肤样本进行分析。结果:UVB暴露显著降低皮肤含水量(-26%),降低胶原蛋白密度,导致表皮和真皮层厚度减少。SVF和ASC治疗改善了皮肤水合作用,增强了细胞外基质重塑,增加了胶原蛋白密度。两种处理都通过降低活性氧(ROS)水平来降低氧化应激,svf处理的样品显示BMAL1表达增加。SVF和ASCs也促进血管生成和淋巴管生成。在SVF和ASC组中也观察到巨噬细胞浸润减少,极化从促炎M1表型向抗炎M2表型转变,ASC表现出更强的免疫调节作用。SVF和ASCs在皮肤组织修复中均表现出明显的治疗优势。结论:这些发现强调了SVF和ASCs作为uvb诱导皮肤衰老的治疗策略的潜力,值得进一步的临床研究。
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来源期刊
CiteScore
6.20
自引率
20.70%
发文量
309
审稿时长
6-12 weeks
期刊介绍: Aesthetic Surgery Journal is a peer-reviewed international journal focusing on scientific developments and clinical techniques in aesthetic surgery. The official publication of The Aesthetic Society, ASJ is also the official English-language journal of many major international societies of plastic, aesthetic and reconstructive surgery representing South America, Central America, Europe, Asia, and the Middle East. It is also the official journal of the British Association of Aesthetic Plastic Surgeons, the Canadian Society for Aesthetic Plastic Surgery and The Rhinoplasty Society.
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