{"title":"Comparative Efficacy of Stromal Vascular Fraction and Adipose-derived Stem Cells in UVB-induced Skin Damage in Nude Mice.","authors":"Hui-Yi Hsiao, Chao-Yi Lai, Yung-Chun Chang, Shih-Han Huang, Jung-Ju Huang","doi":"10.1093/asj/sjaf179","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Chronic UVB exposure accelerates skin aging by inducing oxidative stress and inflammation, leading to dehydration, reduced collagen density, and impaired epidermal and dermal integrity. Stem cell-based therapies, including the use of the stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs), have emerged as promising approaches for skin regeneration.</p><p><strong>Objectives: </strong>This study evaluated the therapeutic effects of SVF and ASCs on UVB-induced skin damage.</p><p><strong>Methods: </strong>The mice were exposed to UVB irradiation (290-310 nm) for eight weeks to establish a photoaging model. Following UVB exposure, the SVF or ASCs were injected into the dorsal skin, and the skin samples were analyzed.</p><p><strong>Results: </strong>UVB exposure significantly reduced skin hydration (-26%), decreased collagen density, and led to a decrease in the thickness of the epidermal and dermal layers. The SVF and ASC treatments improved skin hydration, enhanced extracellular matrix remodeling, and increased collagen density. Both treatments reduced oxidative stress by decreasing reactive oxygen species (ROS) levels, with SVF-treated samples exhibiting increased BMAL1 expression. SVF and ASCs also promoted angiogenesis and lymphangiogenesis. A reduction in macrophage infiltration and a shift in polarization from the proinflammatory M1 phenotype to the anti-inflammatory M2 phenotype were also observed in the SVF and ASC groups, with ASCs exhibiting stronger immunomodulatory effects. The SVF and ASCs each displayed distinct therapeutic advantages in skin tissue restoration.</p><p><strong>Conclusions: </strong>These findings highlight the potential of the SVF and ASCs as therapeutic strategies for UVB-induced skin aging, warranting further clinical investigation.</p>","PeriodicalId":7728,"journal":{"name":"Aesthetic Surgery Journal","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aesthetic Surgery Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/asj/sjaf179","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SURGERY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Chronic UVB exposure accelerates skin aging by inducing oxidative stress and inflammation, leading to dehydration, reduced collagen density, and impaired epidermal and dermal integrity. Stem cell-based therapies, including the use of the stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs), have emerged as promising approaches for skin regeneration.
Objectives: This study evaluated the therapeutic effects of SVF and ASCs on UVB-induced skin damage.
Methods: The mice were exposed to UVB irradiation (290-310 nm) for eight weeks to establish a photoaging model. Following UVB exposure, the SVF or ASCs were injected into the dorsal skin, and the skin samples were analyzed.
Results: UVB exposure significantly reduced skin hydration (-26%), decreased collagen density, and led to a decrease in the thickness of the epidermal and dermal layers. The SVF and ASC treatments improved skin hydration, enhanced extracellular matrix remodeling, and increased collagen density. Both treatments reduced oxidative stress by decreasing reactive oxygen species (ROS) levels, with SVF-treated samples exhibiting increased BMAL1 expression. SVF and ASCs also promoted angiogenesis and lymphangiogenesis. A reduction in macrophage infiltration and a shift in polarization from the proinflammatory M1 phenotype to the anti-inflammatory M2 phenotype were also observed in the SVF and ASC groups, with ASCs exhibiting stronger immunomodulatory effects. The SVF and ASCs each displayed distinct therapeutic advantages in skin tissue restoration.
Conclusions: These findings highlight the potential of the SVF and ASCs as therapeutic strategies for UVB-induced skin aging, warranting further clinical investigation.
期刊介绍:
Aesthetic Surgery Journal is a peer-reviewed international journal focusing on scientific developments and clinical techniques in aesthetic surgery. The official publication of The Aesthetic Society, ASJ is also the official English-language journal of many major international societies of plastic, aesthetic and reconstructive surgery representing South America, Central America, Europe, Asia, and the Middle East. It is also the official journal of the British Association of Aesthetic Plastic Surgeons, the Canadian Society for Aesthetic Plastic Surgery and The Rhinoplasty Society.