Comparative characterization of CRTISO from D. salina and D. bardawil

IF 2.4 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-09-20 DOI:10.1007/s10499-025-02219-x

Jia-Yuan Luo, Qian-Xi Zheng, Yu-Chen Xie, Jv-Liang Dai, Ibrahim Muazzam Mukhtar, Ming-Hua Liang, Hao-Hong Chen, Jian-Guo Jiang

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引用次数: 0

Abstract

Dunaliella, known for its high salt tolerance and photosynthetic efficiency, plays a significant role in industrial and food production. This study presents a comparative analysis of the lycopene biosynthesis pathway in Dunaliella salina and Dunaliella bardawil. Genetic material from both species was extracted and expressed in a bacterial system, followed by HPLC, molecular docking, and phylogenetic analysis. The all-trans-lycopene content in the DsCRT strain was 1.26 ± 0.03 mg/g DCW, higher than that of the DbCRT strain. Molecular docking analysis showed that the binding affinity between DsCRTISO and the substrate was -11.18 kcal/mol, which was lower than the binding affinity of DbCRTISO (-10.08 kcal/mol), revealing that the catalytic activity of DsCRTISO was greater than that of DbCRTISO. Phylogenetic analysis confirmed a close relationship between D. salina and D. bardawil. HPLC showed that D. bardawil accumulate minimal β-carotene, likely due to the failure of specific gene regulatory mechanisms, despite sharing conserved CRTISO domains with D. salina. This study demonstrates that lycopene metabolism enzyme activity in D. salina is significantly higher than in D. bardawil, offering potential for industrial lycopene production.

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盐碱地和巴达维地CRTISO的比较鉴定

Dunaliella以其高耐盐性和光合效率而闻名，在工业和粮食生产中发挥着重要作用。本研究对盐杜氏藻和巴达杜氏藻的番茄红素合成途径进行了比较分析。从两个物种中提取遗传物质并在细菌系统中表达，随后进行HPLC，分子对接和系统发育分析。DsCRT菌株的全反式番茄红素含量为1.26±0.03 mg/g DCW，高于DbCRT菌株。分子对接分析表明，DsCRTISO与底物的结合亲和力为-11.18 kcal/mol，低于DbCRTISO的结合亲和力（-10.08 kcal/mol），表明DsCRTISO的催化活性大于DbCRTISO。系统发育分析证实了D. salina与D. bardawil亲缘关系密切。HPLC显示，尽管与盐藻共享保守的CRTISO结构域，但bardawil积累的β-胡萝卜素很少，可能是由于特定基因调控机制的失败。本研究表明，盐藻的番茄红素代谢酶活性显著高于盐藻，为工业生产番茄红素提供了潜力。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.