A signal-amplifying electrochemical immunosensor based on palladium decorated CoS2 hollow nanoboxes and gold-functionalized Fe3O4 for neuron-specific enolase detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-20 DOI:10.1007/s00604-025-07522-1

Dehao Jia, Yujian Guo, Lei Yang, Xing Gao, Dongquan Leng, Qin Wei

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引用次数: 0

Abstract

A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS₂ hollow nanoboxes (CoS₂-Pd HNBs) as the signal indicator and gold-functionalized Fe₃O₄ (Fe₃O₄-Au) as the EC sensing platform. CoS₂ HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H₂O₂. Moreover, Pd nanoparticles (NPs) were decorated on CoS₂ HNBs by in situ reduction method, which further improved the electrocatalytic performance for H₂O₂ reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe₃O₄ surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab₁) via strong coordination. Fe₃O₄-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.

Graphical abstract

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基于钯修饰CoS2空心纳米盒和金功能化Fe3O4的信号放大电化学免疫传感器用于神经元特异性烯醇化酶检测

利用信号放大技术构建了神经元特异性烯醇化酶（NSE）检测的三明治型电化学（EC）免疫传感器。该策略采用钯装饰CoS2空心纳米盒（CoS2- pd HNBs）作为信号指示器，金功能化Fe3O4 （Fe3O4- au）作为EC传感平台来实现。采用模板法合成了空心结构的CoS2 HNBs，对H2O2表现出优异的电催化性能。此外，通过原位还原的方法将Pd纳米粒子修饰在CoS2 HNBs上，通过协同催化进一步提高了对H2O2还原的电催化性能。在Fe3O4表面均匀修饰Au NPs不仅可以提高EC传感平台的电导率，还可以通过强配位固定捕获抗体（Ab1）。Fe3O4-Au纳米复合材料由于具有良好的磁性能而被固定在磁性玻璃碳电极上，提高了EC免疫传感器的稳定性。值得注意的是，以NSE为目标检测对象的三明治型EC免疫传感器的检出限较低，为0.29 pg/mL (S/N = 3)，线性范围为500 fg/mL至50 ng/mL。这项工作可能为其他生物标志物的检测提供一种有前途的方法。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.