Comparative study of trehalose and glucose in freeze-dried human serum albumin nanocolloids: implications for technetium-99m radiolabeling

IF 1.6 3区化学 Q3 CHEMISTRY, ANALYTICAL

Journal of Radioanalytical and Nuclear Chemistry Pub Date : 2025-09-02 DOI:10.1007/s10967-025-10269-x

Ratna Dini Haryuni, Wening Lestari, Mochammad Dedy Dwi Setyawan, Ega Pratama Hadinoto, Veronika Yulianti Susilo, Amal Rezka Putra, Ahsanal Fikri, Ligwina Dita Pertiwi, Alfian Mahardika Forentin, Sri Setiyowati, Grace Tjungirai Sulungbudi, Ratu Ralna Ismuha, Sumandi Juliyanto

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Abstract

Freeze-drying plays an essential role in creating human serum albumin nanocolloid kits, necessitating the careful choice of stabilizing agents to preserve structural integrity and radiochemical purity after technetium-99 m labeling. This study used trehalose, frequently employed to stabilize proteins during lyophilization, and evaluated its role as a cryoprotectant relative to glucose. Trehalose at 15 mg/mL showed favorable particle size, zeta potential, and radiochemical purity, with stability comparable to glucose. It also offered protection against structural damage. These results support the use of trehalose as a potential alternative cryoprotectant in commercial kits, making it appropriate for use in radiopharmaceutical applications.

Graphical Abstract

Abstract Image

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冻干人血清白蛋白纳米胶体中海藻糖和葡萄糖的比较研究：对锝-99m放射性标记的意义

冷冻干燥在制造人血清白蛋白纳米胶体试剂盒中起着至关重要的作用，需要仔细选择稳定剂，以保持技术-99 m标记后的结构完整性和放射化学纯度。本研究使用海藻糖，通常用于在冻干过程中稳定蛋白质，并评估其作为相对于葡萄糖的冷冻保护剂的作用。15 mg/mL海藻糖具有良好的粒径、zeta电位和放射化学纯度，稳定性与葡萄糖相当。它还提供了防止结构损坏的保护。这些结果支持海藻糖作为商业试剂盒中潜在的替代冷冻保护剂的使用，使其适合用于放射性药物应用。图形抽象

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来源期刊

Journal of Radioanalytical and Nuclear Chemistry 化学-分析化学

CiteScore

2.80

自引率

18.80%

发文量

504

审稿时长

2.2 months

期刊介绍： An international periodical publishing original papers, letters, review papers and short communications on nuclear chemistry. The subjects covered include: Nuclear chemistry, Radiochemistry, Radiation chemistry, Radiobiological chemistry, Environmental radiochemistry, Production and control of radioisotopes and labelled compounds, Nuclear power plant chemistry, Nuclear fuel chemistry, Radioanalytical chemistry, Radiation detection and measurement, Nuclear instrumentation and automation, etc.