Oropharyngeal Microbiome Alterations in Sarcopenia: A Nested Case-Control Study in Older Adults

Abstract

Background Sarcopenia, associated with systemic inflammation, respiratory diseases, and known gut dysbiosis, poses a significant health burden. However, the role of the nasopharyngeal and oropharyngeal (NP/OP) microbiome, a critical respiratory-digestive interface, in its pathogenesis remains unknown. Methods From a cohort of 830 nursing home residents with a sarcopenia prevalence of 61%, we conducted a nested case-control study. Sixty individuals with sarcopenia were propensity-score matched 1:1 with 60 non-sarcopenic controls (N = 120; 50% male). NP/OP swabs underwent full-length 16S rRNA gene sequencing to assess microbial composition and function. Results Individuals with sarcopenia exhibited significantly lower OP microbial α-diversity (Shannon p = 0.016), which remained robust after multivariable adjustment (Shannon p < 0.05). NP diversity was unchanged. Sarcopenia was associated with an NP/OP microbial profile suggesting increased pro-inflammatory potential: enrichment of Moraxella (NP, LDA > 2) and Haemophilus, Lactobacillus amylovorus, Listeriaceae (OP, LDA > 2), alongside depletion of potentially protective taxa (Pasteurellaceae, Alloprevotella tannerae, Prevotella aurantiaca, Eubacterium, Lachnoanaerobaculum) in controls. Specifically, increased Moraxella lincolnii (OR = 1.068, p < 0.05) and decreased Eubacterium (OR = 0.968, p < 0.05) were associated with sarcopenia. Functionally, pathways related to lipopolysaccharide (LPS) biosynthesis and saturated fatty acid metabolism were upregulated in sarcopenia, while short-chain fatty acid (SCFA) and tryptophan metabolism were reduced. Conclusion Oropharyngeal microbial dysbiosis, characterized by lower diversity and a pro-inflammatory signature, is associated with sarcopenia. These findings highlight a potential relationship between the upper respiratory tract microbial environment and sarcopenia, a connection previously underappreciated. Understanding the interplay within the respiratory–gut–muscle axis may offer new perspectives on sarcopenia pathophysiology and its links with respiratory diseases.