Yuting Han , Kai Ye , Geqi Gao , Jinxu Guo , Ming Shan , Donghong Niu
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引用次数: 0
Abstract
Salinity has a significant impact on the growth, energy metabolism, and immune regulation of marine bivalves. To elucidate the response of marine bivalves to low salinity environments and their tolerance mechanisms, this study investigated histopathological alterations and measured oxidative stress indicators along with energy metabolism parameters in gill tissues of Sinonovacula constricta under hyposalinity stress (salinity 5). Transcriptomic profiling of gills from the control group (salinity 20) and low salinity group was concurrently analyzed. The results demonstrated that during the initial stress phase, the antioxidant defense system was activated, evidenced by significant increases in superoxide dismutase (SOD) and catalase (CAT) activities as well as total antioxidant capacity (T-AOC). Concurrently, elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) indicated oxidative stress status. In the later stress phase, declined activities of antioxidant enzymes suggested severe oxidative damage. Throughout hyposalinity exposure, ATP content progressively decreased, while ATPase and pyruvate kinase (PK) activities exhibited an initial increase followed by reduction, reflecting elevated energy expenditure. Transcriptomic analysis identified 501 differentially expressed genes (DEGs) between low salinity and control groups. GO and KEGG enrichment analyses revealed significant enrichment of these DEGs in energy metabolism and protein synthesis-related processes, with notable enrichment in functional enzyme activities associated with the phosphatidylinositol signaling pathway in gills. This study systematically revealed the physiological response mechanisms of S. constricta to hyposalinity stress, and elucidated the roles of the phosphatidylinositol signaling pathway, amino acid metabolism, and endoplasmic reticulum protein processing in the adaptation of S. constricta to hyposalinity stress.
期刊介绍:
Part A: Molecular & Integrative Physiology of Comparative Biochemistry and Physiology. This journal covers molecular, cellular, integrative, and ecological physiology. Topics include bioenergetics, circulation, development, excretion, ion regulation, endocrinology, neurobiology, nutrition, respiration, and thermal biology. Study on regulatory mechanisms at any level of organization such as signal transduction and cellular interaction and control of behavior are also published.