Tobacco ethanol extract accelerates wound healing through anti-inflammation and promoting angiogenesis.

IF 1.3 4区医学 Q3 OPHTHALMOLOGY

Cutaneous and Ocular Toxicology Pub Date : 2025-09-18 DOI:10.1080/15569527.2025.2559680

Fuqin He, Yongjun Liu, Ruiying Yu, Tianli Zhang, Fang Guo, Xiayu Wu, Yi Fan, Xuedong Zhu, Qianyi Zhou, Dan Shu

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引用次数: 0

Abstract

Objective: Wound repair remains a significant challenge in the field of regenerative medicine. Notably, while numerous studies have investigated the effects of isolated nicotine or chlorogenic acid, the systematic evaluation of tobacco ethanol extract (TEE) on cutaneous wound healing has remained uncharted territory. Therefore, this study aims to systematically explore TEE's multifaceted effect on wound healing.

Methods: The hepatorenal toxicity of 5% TEE was evaluated in 6-7-week-old ICR mice. Subsequently, full-thickness 6 mm wounds were created, and images were captured on days 0, 3, 6, 9, and 12. Tissues were collected for H&E staining, Masson trichrome staining, and immunohistochemistry to assess wound healing.

Results: The TEE we prepared showed little hepatorenal toxicity (p > 0.05). By day 12, the wound closure rate in the TEE group reached 95.26%, significantly higher than that in the vehicle group (89.20%, p < 0.05). Histopathological analysis demonstrated that TEE reduced neutrophil infiltration in the wound tissues, accelerated granulation tissue formation, and increased collagen content by 20.99% compared to the vehicle group (p < 0.05). Immunohistochemical staining revealed that on day 6, the positive areas of IL-6 and TNF-α in the TEE group were 5.91% and 4.87% lower than those in the vehicle group (p < 0.05), respectively. On day 9, CD31 expression in the TEE group (8.58%) was significantly higher than that in the vehicle group (5.54%) (p < 0.05).

Conclusion: This study reveals that TEE accelerates wound healing, providing new insights into effective topical therapies for skin wounds while highlighting the potential for tobacco waste valorization.

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烟草乙醇提取物通过抗炎和促进血管生成加速伤口愈合。

目的：创面修复是再生医学领域的一个重大挑战。值得注意的是，虽然已经有大量研究调查了分离尼古丁或绿原酸的影响，但烟草乙醇提取物（TEE）对皮肤伤口愈合的系统评估仍然是未知的领域。因此，本研究旨在系统探讨TEE对伤口愈合的多方面影响。方法：观察5% TEE对6 ~ 7周龄ICR小鼠的肝肾毒性。随后，制作全层6毫米伤口，并在第0、3、6、9和12天拍摄图像。收集组织进行H&E染色、马松三色染色和免疫组织化学评估伤口愈合情况。结果：制备的TEE无明显肝肾毒性（p < 0.05）。到第12天，TEE组创面愈合率达到95.26%，显著高于载药组（89.20%），p p p p结论：本研究揭示了TEE加速创面愈合，为有效的皮肤创面局部治疗提供了新的见解，同时突出了烟草废物价值化的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cutaneous and Ocular Toxicology 医学-毒理学

CiteScore

3.30

自引率

6.20%

发文量

审稿时长

1 months

期刊介绍： Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.