Neuroprotective Effect of Resveratrol Propionate Esters on Apoptosis of SH-SY5Y Cells Induced by Hydrogen Peroxide.

Abstract

Resveratrol, often referred to as 3,4',5-trihydroxystilbene (RSV), is a compound with a variety of pharmacological benefits, such as antiaging, neuroprotective, chemopreventive, antioxidant, and anti-inflammatory qualities. To investigate neuroprotective properties of resveratrol propionate (RPE), H₂O₂ was added to SH-SY5Y cells (human neuroblastoma cell line) that had been pretreated with RPE. The modulation of Bcl-2 and Bax proteins, the mitochondrial membrane potential (MMP), cytochrome c release, the activation of caspase-9 and caspase-3, DNA fragmentation, and membrane catenin analysis were all measured in this study using flow cytometry. The protective effect of RPE pretreatment of SH-SY5Y cells on the H₂O₂-induced death process was further investigated using annexin V-fluorescein isothiocyanate (FITC). The induction of caspase-9 and caspase-3, release of cytochrome c, loss of MMP, modification of Bcl-2 and Bax, DNA fragmentation, and annexin V-FITC/PI (propidium iodide) double staining all indicate that RPEs are capable of successfully shielding SH-SY5Y cells from H₂O₂-induced cytotoxicity. Therefore, RPE is considered a good candidate for the prevention and treatment of neurodegeneration induced by oxidative damage.