Interleukin-37 Ameliorates Articular Cartilage Damage in Two Murine Models of Osteoarthritis.

IF 2.7 4区 医学 Q1 ORTHOPEDICS
Ellen W van Geffen, Henk M van Beuningen, Joyce Aarts, Elly L Vitters, Wim H C Rijnen, Arjen B Blom, Fons A J van de Loo, Esmeralda N Blaney Davidson, Marije I Koenders, Arjan P M van Caam, Peter M van der Kraan
{"title":"Interleukin-37 Ameliorates Articular Cartilage Damage in Two Murine Models of Osteoarthritis.","authors":"Ellen W van Geffen, Henk M van Beuningen, Joyce Aarts, Elly L Vitters, Wim H C Rijnen, Arjen B Blom, Fons A J van de Loo, Esmeralda N Blaney Davidson, Marije I Koenders, Arjan P M van Caam, Peter M van der Kraan","doi":"10.1177/19476035251372304","DOIUrl":null,"url":null,"abstract":"<p><p>ObjectiveIn this study, we investigated whether interleukin (IL)-37 ameliorates experimental osteoarthritis (OA).MethodsThe effects of IL-37 were investigated in collagenase-induced OA (CiOA) and destabilization of the medial meniscus (DMM). Human IL-37-adenovirus (ad-IL-37) was injected into the affected knee joint 4 and 18 days after the induction of OA. Luciferase-adenovirus was injected as control. Mice were sacrificed for histology at early and late stage of OA development. Interleukin-37 protein expression was confirmed by immunohistochemistry. Cartilage damage, osteophyte size and joint capsule thickness were measured. The effectiveness of ad-IL-37 was confirmed in vitro in human OA fibroblasts using real-time qualitative polymerase chain reaction (RT-qPCR) and Western blotting.ResultsInterleukin-37 protein expression was visible for at least 7 days after injection. At day 28, 10 days after the second injection, no clear synovial IL-37 staining was found any more, in both models. At day 28 of CiOA, ad-IL-37 significantly reduced articular cartilage damage and this was still reduced, although not significantly, at the late time point (day 42). In the DMM model, significant beneficial effect of IL-37 on cartilage damage was found at the late time point. In both OA models ad-IL-37 injections reduced the size of osteophytes.ConclusionInterleukin-37 ameliorated OA-induced articular cartilage damage and osteophyte formation in both models.</p>","PeriodicalId":9626,"journal":{"name":"CARTILAGE","volume":" ","pages":"19476035251372304"},"PeriodicalIF":2.7000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449308/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"CARTILAGE","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/19476035251372304","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ORTHOPEDICS","Score":null,"Total":0}
引用次数: 0

Abstract

ObjectiveIn this study, we investigated whether interleukin (IL)-37 ameliorates experimental osteoarthritis (OA).MethodsThe effects of IL-37 were investigated in collagenase-induced OA (CiOA) and destabilization of the medial meniscus (DMM). Human IL-37-adenovirus (ad-IL-37) was injected into the affected knee joint 4 and 18 days after the induction of OA. Luciferase-adenovirus was injected as control. Mice were sacrificed for histology at early and late stage of OA development. Interleukin-37 protein expression was confirmed by immunohistochemistry. Cartilage damage, osteophyte size and joint capsule thickness were measured. The effectiveness of ad-IL-37 was confirmed in vitro in human OA fibroblasts using real-time qualitative polymerase chain reaction (RT-qPCR) and Western blotting.ResultsInterleukin-37 protein expression was visible for at least 7 days after injection. At day 28, 10 days after the second injection, no clear synovial IL-37 staining was found any more, in both models. At day 28 of CiOA, ad-IL-37 significantly reduced articular cartilage damage and this was still reduced, although not significantly, at the late time point (day 42). In the DMM model, significant beneficial effect of IL-37 on cartilage damage was found at the late time point. In both OA models ad-IL-37 injections reduced the size of osteophytes.ConclusionInterleukin-37 ameliorated OA-induced articular cartilage damage and osteophyte formation in both models.

白细胞介素-37改善两种小鼠骨关节炎模型的关节软骨损伤。
目的探讨白细胞介素(IL)-37是否能改善实验性骨关节炎(OA)。方法观察IL-37在胶原酶诱导的骨关节炎(CiOA)和内侧半月板失稳(DMM)中的作用。人il -37腺病毒(ad-IL-37)在OA诱导后4天和18天注射到患膝关节。注射荧光素酶腺病毒作为对照。在OA发展的早期和晚期处死小鼠进行组织学观察。免疫组化法证实白细胞介素-37蛋白表达。测量软骨损伤、骨赘大小和关节囊厚度。利用实时定性聚合酶链反应(RT-qPCR)和Western blotting验证了ad-IL-37在体外对人OA成纤维细胞的有效性。结果注射后至少7 d可见白介素-37蛋白的表达。第二次注射后第28天、第10天,两种模型滑膜IL-37均未见明显染色。在CiOA的第28天,ad-IL-37显著减少了关节软骨损伤,在后期(第42天),这种损伤仍然减少,尽管不明显。在DMM模型中,IL-37在较晚时间点对软骨损伤有明显的有益作用。在两种OA模型中,注射ad-IL-37可减小骨赘的大小。结论白细胞介素-37可改善oa诱导的关节软骨损伤和骨赘形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CARTILAGE
CARTILAGE ORTHOPEDICS-
CiteScore
6.90
自引率
7.10%
发文量
80
期刊介绍: CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信