Cartilage Redox Balance Is Influenced by Substrate Availability.

IF 2.7 4区医学 Q1 ORTHOPEDICS

CARTILAGE Pub Date : 2025-09-19 DOI:10.1177/19476035251377532

Jingyi Wang, Corinne R Henak

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引用次数: 0

Abstract

PurposeThe mechanobiological response of cartilage redox balance might change in response to altered substrate availability. The purpose of this study is to investigate effects of key metabolic substrates (glucose, glutamine, and oxygen) on the mechanoresponsiveness of cartilage redox balance using a label-free imaging technique that measures autofluorescence from endogenous redox cofactors.FindingsCompared with room oxygen tension, low oxygen tension had higher autofluorescence intensity in green (FAD) channel after a single sub-failure tensile load. Cartilage explants cultured in high glucose medium with glutamine supply had higher autofluorescence intensity in both channels (FAD and NADH/NADPH) compared with low glucose with glutamine group or high glucose without glutamine group, while no difference was observed between the latter 2 groups.ConclusionsLow oxygen tension or high glucose culture medium with glutamine supply increases the mechanoresponsiveness of cartilage redox balance induced by sub-failure mechanical loading. Glutamine appears to partially serve as a glucose substitute in this process.

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软骨氧化还原平衡受底物有效性的影响。

目的软骨氧化还原平衡的力学生物学反应可能随着底物可利用性的改变而改变。本研究的目的是研究关键代谢底物（葡萄糖、谷氨酰胺和氧）对软骨氧化还原平衡机械反应性的影响，使用无标记成像技术测量内源性氧化还原辅助因子的自身荧光。结果与室内氧张力相比，低氧张力在单次亚失效拉伸载荷后具有更高的绿色（FAD）通道自身荧光强度。与低糖加谷氨酰胺组和高糖不加谷氨酰胺组相比，高糖加谷氨酰胺组软骨外植体在FAD和NADH/NADPH两个通道上的自身荧光强度更高，后两组间无显著差异。结论低氧张力或高糖培养基中添加谷氨酰胺可增加亚失效机械负荷诱导的软骨氧化还原平衡的机械反应性。在这个过程中，谷氨酰胺似乎部分地充当了葡萄糖的替代品。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.