Laser-induced WO3-decorated porous graphene for portable and self-powered photoelectrochemical aptasensing

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-19 DOI:10.1007/s00604-025-07532-z

Lulu Zhang, Lingbo Liu, Jiang Guo, Jingdong Zhang, Kai Yan

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引用次数: 0

Abstract

Photoelectrochemical (PEC) biosensors have attracted significant interest due to their inherent advantages of high sensitivity, excellent biocompatibility, and ease of miniaturization. In this study, efficient WO₃-based photoelectrodes were developed via a novel one-step laser-scribing strategy, enabling the in-situ synthesis of patterned, flexible WO₃-decorated porous graphene electrodes (LIG-WO₃) using H₂WO₄-coated polyimide (PI) film as the precursor. The localized high temperature and reducing atmosphere generated during laser irradiation simultaneously convert the PI into laser-induced graphene and decompose H₂WO₄, yielding photoactive WO₃ and conductive metallic W nanoparticles embedded in the porous graphene matrix. The optimized LIG-WO₃ photoelectrode exhibits a rapid photoelectric response under visible Light illumination at 0 V applied bias, enabling self-powered PEC sensing. A visible light-driven, self-powered PEC aptasensor was fabricated by immobilizing an oxytetracycline (OTC) specific aptamer onto the photoelectrode surface. The Sensor exhibits a Linear response toward OTC in the range 1.0 -1.0 × 10³ nM, with a low detection Limit of 0.68 nM (S/N = 3) as well as high stability, reproducibility and applicability in real sample analysis. This work demonstrates the significant promise of one-step laser-induced WO₃ electrodes for advancing self-powered PEC sensing applications.

Graphical Abstract

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激光诱导wo3修饰的多孔石墨烯用于便携式自供电光电电化学感应。

光电化学（PEC）生物传感器以其高灵敏度、良好的生物相容性和易于小型化等优点而受到人们的广泛关注。在这项研究中，通过一种新的一步激光刻划策略，开发了高效的wo3基光电极，使原位合成有图案的、柔性的wo3修饰的多孔石墨烯电极（ligo - wo3）成为可能，该电极使用h2wo4涂层聚酰亚胺（PI）薄膜作为前驱体。激光照射过程中产生的局部高温和还原气氛同时将PI转化为激光诱导的石墨烯，并分解H2WO4，生成光活性WO3和嵌入多孔石墨烯基体中的导电金属W纳米颗粒。优化后的ligo - wo3光电极在0 V偏置可见光照射下表现出快速的光电响应，实现了自供电的PEC传感。通过将土霉素（OTC）特异性适配体固定在光电极表面，制备了一种可见光驱动、自供电的PEC适配体传感器。该传感器在1.0 ~ 1.0 × 103 nM范围内对OTC具有良好的线性响应，检测限低至0.68 nM (S/N = 3)，具有较高的稳定性、重现性和在实际样品分析中的适用性。这项工作证明了一步激光诱导WO3电极在推进自供电PEC传感应用方面的重大前景。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.