An endoplasmic reticulum-targeted fluorescent probe for detecting polarity and viscosity and its bioimaging in vitro and vivo

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-09-19 DOI:10.1016/j.snb.2025.138803

Jun-Tao Niu , Yi-Ru Wang , Chen-Chen Li , Hai-Feng Yin , Qian Xu , Ting Liang , Xu-Ying Liu , Yan-Fei Kang

{"title":"An endoplasmic reticulum-targeted fluorescent probe for detecting polarity and viscosity and its bioimaging in vitro and vivo","authors":"Jun-Tao Niu , Yi-Ru Wang , Chen-Chen Li , Hai-Feng Yin , Qian Xu , Ting Liang , Xu-Ying Liu , Yan-Fei Kang","doi":"10.1016/j.snb.2025.138803","DOIUrl":null,"url":null,"abstract":"<div><div>Revealing the dynamics of intracellular microenvironment (viscosity and polarity) during endoplasmic reticulum (ER) stress is crucial for the diagnosis of related diseases. Herein, we developed an ER-targeted dual-response fluorescent probe (<strong>XHXA</strong>) for simultaneously detecting polarity and viscosity with large emission spectral separation (>180 nm). By leveraging intramolecular charge transfer (ICT) and twisted intramolecular charge transfer (TICT) mechanisms, <strong>XHXA</strong> exhibited excellent responsiveness to polarity and viscosity at emission wavelengths of 482 nm and 665 nm, respectively. Moreover, <strong>XHXA</strong> enabled real-time imaging of ER microenvironment in living cells, and tracking ER stress and autophagy. Meanwhile, reduced polarity and increased viscosity were observed in non-alcoholic fatty liver disease (NAFLD) models via <strong>XHXA</strong>. Notably, <strong>XHXA</strong> was first applied to diagnose liver cirrhosis in vivo through monitoring ER viscosity. Thus, this work established a powerful platform for early diagnostics and investigating the physiological and pathological of disease related with ER stress.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"447 ","pages":"Article 138803"},"PeriodicalIF":3.7000,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925400525015795","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Revealing the dynamics of intracellular microenvironment (viscosity and polarity) during endoplasmic reticulum (ER) stress is crucial for the diagnosis of related diseases. Herein, we developed an ER-targeted dual-response fluorescent probe (XHXA) for simultaneously detecting polarity and viscosity with large emission spectral separation (>180 nm). By leveraging intramolecular charge transfer (ICT) and twisted intramolecular charge transfer (TICT) mechanisms, XHXA exhibited excellent responsiveness to polarity and viscosity at emission wavelengths of 482 nm and 665 nm, respectively. Moreover, XHXA enabled real-time imaging of ER microenvironment in living cells, and tracking ER stress and autophagy. Meanwhile, reduced polarity and increased viscosity were observed in non-alcoholic fatty liver disease (NAFLD) models via XHXA. Notably, XHXA was first applied to diagnose liver cirrhosis in vivo through monitoring ER viscosity. Thus, this work established a powerful platform for early diagnostics and investigating the physiological and pathological of disease related with ER stress.

Abstract Image

查看原文本刊更多论文

一种内质网定向荧光探针，用于检测极性和粘度及其在体外和体内的生物成像

揭示内质网（ER）应激过程中细胞内微环境（粘度和极性）的动态变化对相关疾病的诊断至关重要。在此，我们开发了一种er靶向双响应荧光探针（XHXA），用于同时检测极性和粘度，具有大发射光谱分离（>180 nm）。通过利用分子内电荷转移（ICT）和扭曲分子内电荷转移（TICT）机制，XHXA分别在482 nm和665 nm发射波长下对极性和粘度表现出优异的响应性。此外，XHXA能够实时成像活细胞内质网微环境，跟踪内质网应激和自噬。同时，在非酒精性脂肪性肝病（NAFLD）模型中，通过XHXA观察到极性降低和粘度增加。值得注意的是，XHXA首次在体内通过监测内质网黏度来诊断肝硬化。因此，这项工作为早期诊断和研究内质网应激相关疾病的生理和病理建立了强大的平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.