Hmong medicine tooth-fixing formula promotes bone repair in periodontitis

IF 8.3 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-09-11 DOI:10.1016/j.phymed.2025.157255

Ming Dong , Shuo Liu , Haibo Sun, Meichen Tong, Qianyang Chen, Shuixiang Guo, Gege Zhang, Chun Shi, Lina Wang, Weidong Niu

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引用次数: 0

Abstract

Background

Periodontitis is the number one cause of adult tooth loss worldwide. The main treatments for periodontitis are removed tartar, surgery, and medication, but none of them can bring about a cure. Traditional Chinese medicine as an adjunct to modulation of the host immune response in the treatment of periodontitis needs to be further researched and explored.

Objective

This study investigated the effect of Hmong Medicine Tooth-fixing Formula (HM-TF) on periosteal bone repair in vivo and in vitro to provide an experimental basis for the use of the Hmong medicine in the clinical treatment of periodontitis.

Methods

Screening of HM-TF and periodontitis co-action targets by network pharmacology analysis. The effects of HM-TF on the proliferation and differentiation of osteoblasts and osteoclasts were investigated by CCK-8 assay, Real-time qPCR, western blotting, ALP, alizarin red S, and TRAP staining. A rat periodontitis model was established, and micro-computed tomography (CT) and hematoxylin and eosin (HE) staining experiments were carried out to study the effect of HM-TF on bone repair and investigate its biosafety.

Results

The core active ingredients of HM-TF for treating periodontitis include quercetin, luteolin, kaempferol, etc. One of the main targets of HM-TF in periodontitis is RXRα, and all the active ingredients can effectively bind to RXRα. HM-TF enhanced the growth of osteoblasts by increasing the production of RXRα and factors related to bone formation. Additionally, it hindered the development of osteoclasts by reducing the expression of factors related to osteoclasts. Three-dimensional (3D) micro-CT, HE staining and immunohistochemical staining showed that HM-TF ameliorated the alveolar bone destruction caused by periodontitis in rats, and the effect was better than that of metronidazole.

Conclusion

One of the main intersecting targets of HM-TF and periodontitis is RXRα. HM-TF promoted bone repair in rats with periodontitis and had good biocompatibility.

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苗族药物固牙配方促进牙周炎患者的骨修复。

背景：牙周炎是全球成年人牙齿脱落的头号原因。牙周炎的主要治疗方法是除牙垢、手术和药物治疗，但没有一种方法能治愈牙周炎。中医药辅助调节宿主免疫反应治疗牙周炎的研究有待进一步探索。目的：研究苗族药物固牙方（HM-TF）对骨膜骨的体内外修复作用，为苗族药物在牙周炎临床治疗中的应用提供实验依据。方法：采用网络药理学方法筛选牙周炎联合作用靶点。采用CCK-8、Real-time qPCR、western blotting、ALP、茜素红S、TRAP染色等方法观察汉霉素对成骨细胞和破骨细胞增殖分化的影响。建立大鼠牙周炎模型，通过显微计算机断层扫描（CT）和苏木精伊红（HE）染色实验研究HM-TF对骨修复的影响及生物安全性。结果：中药复方治疗牙周炎的核心有效成分包括槲皮素、木犀草素、山奈酚等。tm - tf治疗牙周炎的主要靶点之一是RXRα，所有有效成分均能与RXRα有效结合。HM-TF通过增加RXRα和骨形成相关因子的产生促进成骨细胞的生长。此外，它通过降低破骨细胞相关因子的表达来阻碍破骨细胞的发育。三维（3D）显微ct、HE染色和免疫组织化学染色显示，HM-TF能改善大鼠牙周炎所致的牙槽骨破坏，且效果优于甲硝唑。结论：RXRα是HM-TF与牙周炎的主要交叉靶点之一。HM-TF促进牙周炎大鼠骨修复，具有良好的生物相容性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.