{"title":"Long-Wavelength Emitting Fluorescent Probe for Real-Time Peroxynitrite Imaging in Living Cells.","authors":"Shaozhu Li, Yanmeng Zhu, Qi Dong, Shuqi Xiong, Rufang Zhang, Jiajia Yu, Mengying Zhang, Jun Tang","doi":"10.1007/s10895-025-04484-8","DOIUrl":null,"url":null,"abstract":"<p><p>Peroxynitrite (ONOO<sup>-</sup>), a highly reactive nitrogen species with potent oxidative capacity, plays dual roles in cellular signaling and disease pathogenesis. To address the limitations of existing ONOO<sup>-</sup> probes-particularly poor aqueous solubility and inadequate emission wavelengths-we developed P<sub>30</sub>, a novel water-soluble fluorescent probe based on a rigid molecular scaffolding. P<sub>30</sub> selectively detects ONOO<sup>-</sup> through azo-bond oxygenolysis, triggering rapid (< 10 s), sensitive (LOD = 0.38 µM), and long-wavelength fluorescence emission (λ<sub>em</sub> = 612 nm). Crucially, P<sub>30</sub> enables real-time visualization of both exogenous and endogenous ONOO<sup>-</sup> in live cells, demonstrating its utility for interrogating ONOO<sup>-</sup> dynamics in biological systems. These attributes position P<sub>30</sub> as a robust tool for studying ONOO<sup>-</sup>-associated pathophysiological processes.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04484-8","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Peroxynitrite (ONOO-), a highly reactive nitrogen species with potent oxidative capacity, plays dual roles in cellular signaling and disease pathogenesis. To address the limitations of existing ONOO- probes-particularly poor aqueous solubility and inadequate emission wavelengths-we developed P30, a novel water-soluble fluorescent probe based on a rigid molecular scaffolding. P30 selectively detects ONOO- through azo-bond oxygenolysis, triggering rapid (< 10 s), sensitive (LOD = 0.38 µM), and long-wavelength fluorescence emission (λem = 612 nm). Crucially, P30 enables real-time visualization of both exogenous and endogenous ONOO- in live cells, demonstrating its utility for interrogating ONOO- dynamics in biological systems. These attributes position P30 as a robust tool for studying ONOO--associated pathophysiological processes.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
