Genetic parameters, genome-wide associations and potential candidate genes for additive and dominance effects of tail traits in Merinoland sheep based on whole-genome sequence data in a selection experiment

IF 2.1 3区 生物学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal genetics Pub Date : 2025-09-18 DOI:10.1111/age.70041
Johanna Mainzer, Tong Yin, Isabella Giambra, Hannah Hümmelchen, Petra Engel, Henrik Wagner, Axel Wehrend, Sven König
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Abstract

The aim of this study was an in-depth genomic analysis for tail length (TL), tail characteristics and body measurements in the Merinoland sheep breed considering whole-genome sequence data. Genomic analyses included the estimation of genetic parameters and dominance effects, genome-wide associations for the additive and dominance component, and the annotation of potential candidate genes. We implemented a unified selection and mating experiment to create extreme lamb groups based on breeding values for TL. The 254 lambs from the mating experiment were phenotyped at birth for TL, tail circumference, and body length (all in cm), for body weight, and X-rayed to count the number of vertebrae and to identify tail abnormalities for tail fractures, axis deviations, block vertebrae, and wedged vertebrae. Heritabilities using the variant-based relationship matrix were large for the morphological measurements TL (0.85), body length (0.93), and body weight (0.85), moderate for tail circumference (0.21), and number of vertebrae (0.29), but close to zero for tail abnormalities. Dominance variance for TL explained 14.95% of the phenotypic variation, but was close to zero for the remaining tail traits. The positive breeding value correlations indicate longer and thicker tails for taller and heavier lambs. Breeding value correlations were negative between TL with block vertebrae and wedged vertebrae. Genome-wide associations for additive-genetic and dominance effects revealed 726 significant variants, which are located close to potential candidate genes. These candidate genes have known functions on skeletal growth, and regulate the development of bone structures and of vertebrae characteristics.

Abstract Image

基于全基因组序列数据的美利奴羊尾部性状加性和显性效应的遗传参数、全基因组关联及潜在候选基因选择实验
本研究的目的是利用全基因组序列数据对美利诺羊品种的尾长、尾特征和体测量进行深入的基因组分析。基因组分析包括遗传参数和显性效应的估计,加性和显性成分的全基因组关联,以及潜在候选基因的注释。我们采用统一的选择和交配实验,以TL育种值为基础创建极端羔羊群。交配实验的254只羔羊在出生时进行TL、尾围、体长(均以cm为单位)和体重表型分析,并进行x光检查,计算椎骨数量,并识别尾巴骨折、轴偏、椎骨块和楔状椎骨等尾巴异常。变异关系矩阵对形态测量TL(0.85)、体长(0.93)和体重(0.85)的遗传力较大,对尾围(0.21)和椎骨数(0.29)的遗传力中等,但对尾巴异常的遗传力接近于零。TL显性方差解释了14.95%的表型变异,其余尾部性状的显性方差接近于零。正育种值相关性表明,较高、较重的羔羊尾巴较长、较粗。块状椎体与楔状椎体的TL育种值呈负相关。加性遗传和显性效应的全基因组关联揭示了726个显著变异,这些变异位于潜在候选基因附近。这些候选基因在骨骼生长中具有已知的功能,并调节骨结构和椎骨特征的发育。
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来源期刊
Animal genetics
Animal genetics 生物-奶制品与动物科学
CiteScore
4.60
自引率
4.20%
发文量
115
审稿时长
5 months
期刊介绍: Animal Genetics reports frontline research on immunogenetics, molecular genetics and functional genomics of economically important and domesticated animals. Publications include the study of variability at gene and protein levels, mapping of genes, traits and QTLs, associations between genes and traits, genetic diversity, and characterization of gene or protein expression and control related to phenotypic or genetic variation. The journal publishes full-length articles, short communications and brief notes, as well as commissioned and submitted mini-reviews on issues of interest to Animal Genetics readers.
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