Effect of electroporation on neuronal excitability under H-FIRE pulses

IF 4.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-09-13 DOI:10.1016/j.bioelechem.2025.109109

Fei Guo, Li Luo, Chunhuai Gong, Kai Pei

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Abstract

The traditional Hodgkin-Huxley (HH) model is mainly applicable to neuronal excitability under low electric fields but ails at high field intensities. In this study, an improved HH model incorporating electroporation (EP) current was proposed. Simulation results showed that under a classical IRE pulse (100 μs, 440 V/cm), EP current evoked an action potential (AP) with a peak of 18.38 mV at t = 2.98 ms; whereas under a burst of H-FIRE pulses (1–1-1-1 μs, 440 V/cm), the AP peak decreased to 13.05 mV (by 5.33 mV) and was delayed to t = 26.82 ms. Further analysis revealed that both IRE and H-FIRE stimulation have an optimal electric field window: moderate increases in field strength enhanced excitability, while excessive intensity caused inhibitory effects due to over-electroporation. In addition, prolonging the inter-phase delay, inter-pulse delay, and pulse width of H-FIRE pulses aggravated EP effects and significantly suppressed excitability. Compared with the classical HH model, the proposed model more accurately reflects neuronal excitability under high electric fields and has important implications for the study of single-neuron stimulation.

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H-FIRE脉冲下电穿孔对神经元兴奋性的影响。

传统的霍奇金-赫胥黎（HH）模型主要适用于低电场下的神经元兴奋性，而不适用于高场强下的神经元兴奋性。在这项研究中，提出了一个改进的HH模型，其中包括电穿孔（EP）电流。仿真结果表明，在经典IRE脉冲（100 μs, 440 V/cm）下，EP电流在t = 2.98 ms时产生的动作电位峰值为18.38 mV；而在H-FIRE脉冲（1-1-1-1 μs, 440 V/cm）下，AP峰值降低到13.05 mV（降低5.33 mV），延迟到t = 26.82 ms。进一步分析表明，IRE和H-FIRE刺激均有一个最佳电场窗口：适度增加电场强度可增强兴奋性，而强度过大则会因过度电穿孔而产生抑制作用。延长H-FIRE脉冲的相间延迟、脉冲间延迟和脉宽可加重EP效应，显著抑制兴奋性。与经典HH模型相比，该模型更准确地反映了高电场下神经元的兴奋性，对单神经元刺激的研究具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.