{"title":"Mapping rRNA, tRNA, and mRNA modifications in ribosomes at high resolution.","authors":"Łukasz Koziej, Sebastian Glatt","doi":"10.1016/j.tibs.2025.08.007","DOIUrl":null,"url":null,"abstract":"<p><p>Chemical modifications of rRNA, tRNA, and mRNA play key roles in protein synthesis by affecting the structure of these RNAs, by modulating decoding, and by influencing ribosomal efficiency. Recent advances in sequencing-based detection methods have expanded our ability to map these moieties across RNA molecules in diverse cellular states. In parallel, X-ray crystallography and the advent of high-resolution cryogenic electron microscopy have facilitated the direct visualization of RNA modifications within ribosomes. This review integrates recent structural studies with functional insights to shed light on the roles of RNA modifications in translation. Thereby, we seek to summarize current knowledge about the molecular roles of RNA modifications in gene expression and protein synthesis.</p>","PeriodicalId":440,"journal":{"name":"Trends in Biochemical Sciences","volume":" ","pages":""},"PeriodicalIF":11.0000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Biochemical Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.tibs.2025.08.007","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Chemical modifications of rRNA, tRNA, and mRNA play key roles in protein synthesis by affecting the structure of these RNAs, by modulating decoding, and by influencing ribosomal efficiency. Recent advances in sequencing-based detection methods have expanded our ability to map these moieties across RNA molecules in diverse cellular states. In parallel, X-ray crystallography and the advent of high-resolution cryogenic electron microscopy have facilitated the direct visualization of RNA modifications within ribosomes. This review integrates recent structural studies with functional insights to shed light on the roles of RNA modifications in translation. Thereby, we seek to summarize current knowledge about the molecular roles of RNA modifications in gene expression and protein synthesis.
期刊介绍：
For over 40 years, Trends in Biochemical Sciences (TIBS) has been a leading publication keeping readers informed about recent advances in all areas of biochemistry and molecular biology. Through monthly, peer-reviewed issues, TIBS covers a wide range of topics, from traditional subjects like protein structure and function to emerging areas in signaling and metabolism. Articles are curated by the Editor and authored by top researchers in their fields, with a focus on moving beyond simple literature summaries to providing novel insights and perspectives. Each issue primarily features concise and timely Reviews and Opinions, supplemented by shorter articles including Spotlights, Forums, and Technology of the Month, as well as impactful pieces like Science & Society and Scientific Life articles.
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