Effects of Angelica sinensis polysaccharides on growth performance, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in Mauremys mutica

IF 2.4 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-09-19 DOI:10.1007/s10499-025-02240-0

Zike Yan, Jingu Shi, Kaijie Lin, Yuhan Li, Yutian Zhan, Mingzuo Gao, Hongyao Zhang, Shuyu Han, Jingzhen Liang

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引用次数: 0

Abstract

The yellow pond turtle (Mauremys mutica), an important freshwater species in Chinese aquaculture, faces increasing threats from bacterial diseases such as Klebsiella pneumoniae, necessitating safe immunostimulatory strategies. Angelica sinensis polysaccharides (ASP), with immunomodulatory, antioxidant, and metabolic regulatory properties, are a promising feed additive. This study evaluated dietary ASP effects on growth, antioxidant capacity, immune response, intestinal microbiota, and liver transcriptome in M. mutica. A total of 540 turtles (150.42 ± 5.61 g) were randomly assigned to a control group (fed with basal diet, denoted as A0) and five experimental groups supplemented with 0.05%, 0.10%, 0.20%, 0.40%, and 0.80% ASP (A5, A10, A20, A40, and A80) for 65 days. ASP significantly reduced hepatosomatic index (HSI) (P = 0.001) and enhanced hepatic antioxidant enzyme activities, including superoxide dismutase (SOD) (P = 0.049), total antioxidant capacity (T-AOC) (P = 0.003), and glutathione peroxidase (GSH-PX) (P = 0.026); lowered malondialdehyde (MDA) (P = 0.002); decreased serum total cholesterol (TC) (P = 0.009) and triglycerides (TG) (P = 0.006); and elevated serum immune Markers, including complement component 4 (C4) (P < 0.001), acid phosphatase (ACP) (P = 0.011), alkaline phosphatase (AKP) (P < 0.001), and lysozyme (LZM) (P < 0.001). Regression analysis indicated an optimal supplementation of 0.17–0.28%. Following K. pneumoniae challenge, A20 showed the highest survival rate (73.33%) and relative percent survival (50.00%) (P = 0.002). ASP also increased beneficial intestinal taxa (Lachnospira, Muribaculaceae_unclassified, Anaerovibrio) and activated immune-related liver pathways, including hematopoietic cell lineage, pattern recognition receptors, and cell adhesion molecules, with key genes such as HLA-DRA, CSMD1, and CLEC2D. These findings highlight ASP as a functional feed additive for improving immunity and health in M. mutica aquaculture.

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当归多糖对毛鼠生长性能、抗氧化能力、免疫反应、肠道菌群和肝脏转录组的影响

黄塘龟（Mauremys mutica）是我国水产养殖中的重要淡水物种，其面临肺炎克雷伯菌等细菌性疾病的威胁日益严重，需要采取安全的免疫刺激措施。当归多糖（ASP）具有免疫调节、抗氧化和代谢调节等特性，是一种很有前途的饲料添加剂。本研究评估了饲粮中ASP对mutica m.s生长、抗氧化能力、免疫反应、肠道微生物群和肝脏转录组的影响。选取540只（150.42±5.61 g）海龟，随机分为对照组（饲喂基础饲粮，用A0表示）和5个试验组(分别添加0.05%、0.10%、0.20%、0.40%和0.80% ASP (A5、A10、A20、A40和A80)，试验期65 d。ASP显著降低肝体指数（HSI）（P = 0.001），提高肝脏抗氧化酶活性，包括超氧化物歧化酶（SOD）（P = 0.049）、总抗氧化能力（T-AOC）（P = 0.003）和谷胱甘肽过氧化物酶（GSH-PX）（P = 0.026）；丙二醛（MDA）降低（P = 0.002）；血清总胆固醇（TC）（P = 0.009）和甘油三酯（TG）（P = 0.006）降低；血清免疫指标升高，包括补体成分4 (C4) （P < 0.001）、酸性磷酸酶（ACP）（P = 0.011）、碱性磷酸酶（AKP）（P < 0.001）和溶菌酶（LZM）（P < 0.001）。回归分析表明，最佳添加量为0.17 ~ 0.28%。肺炎克雷伯菌攻毒后，A20的存活率最高（73.33%），相对存活率最高（50.00%）（P = 0.002）。ASP还增加了有益的肠道类群（毛螺旋菌，muribaculacae_unclassified，厌氧弧菌），并激活了免疫相关的肝脏通路，包括造血细胞谱系，模式识别受体和细胞粘附分子，关键基因如HLA-DRA， CSMD1和cle2d。这些研究结果表明，ASP可作为一种功能性饲料添加剂，在提高黄颡鱼养殖的免疫力和健康方面具有重要作用。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.