Signaling-dependent refinement of cell fate choice during tissue remodeling in Drosophila pupal wings

IF 8.7 1区生物学 Q1 CELL BIOLOGY

Developmental cell Pub Date : 2025-09-18 DOI:10.1016/j.devcel.2025.08.016

Sophie Herszterg, Simone Cicolini, Marc de Gennes, Anqi Huang, Alexis Matamoro-Vidal, Cyrille Alexandre, Matthew Smith, Helena Araujo, Romain Levayer, Jean-Paul Vincent, Guillaume Salbreux

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Abstract

How cell fate decisions and tissue remodeling are coordinated to establish precise and robust patterns is a fundamental question in developmental biology. Here, we investigate this interplay during the refinement of Drosophila wing veins. We show by live imaging that vein refinement is driven initially by local tissue deformation, followed by cell fate adjustments orchestrated by a signaling network involving Notch, EGFR, and Dpp. Dynamic tracking of signaling reporter activity uncovers a wave of Notch signaling that converts wide crude proveins into thin stereotypical veins. Perturbing large-scale convergence and extension does not affect vein refinement, and optogenetically induced veins refine irrespective of their orientation, demonstrating that the signaling network suffices for refinement, independently of large-scale tissue flows. A minimal biophysical description recapitulates the signaling network’s ability to coordinate vein refinement in various experimental situations. Our results illustrate how cell fate decisions are updated for robust patterning in a remodeling tissue.

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果蝇蛹翅组织重塑过程中细胞命运选择的信号依赖细化

细胞命运决定和组织重塑如何协调建立精确和稳健的模式是发育生物学的一个基本问题。在这里，我们研究了果蝇翼静脉细化过程中的这种相互作用。我们通过实时成像显示，静脉细化最初是由局部组织变形驱动的，随后是由Notch、EGFR和Dpp等信号网络协调的细胞命运调整。信号报告者活动的动态跟踪揭示了Notch信号的一波，它将宽的粗静脉转化为细的典型静脉。干扰大范围的收敛和延伸不会影响静脉的细化，而光遗传诱导的静脉细化与它们的方向无关，这表明信号网络足以细化，独立于大规模的组织流动。一个最小的生物物理描述概括了信号网络在各种实验情况下协调静脉细化的能力。我们的研究结果说明了细胞命运决定是如何在重塑组织中更新的。

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来源期刊

Developmental cell 生物-发育生物学

CiteScore

18.90

自引率

1.70%

发文量

203

审稿时长

3-6 weeks

期刊介绍： Developmental Cell, established in 2001, is a comprehensive journal that explores a wide range of topics in cell and developmental biology. Our publication encompasses work across various disciplines within biology, with a particular emphasis on investigating the intersections between cell biology, developmental biology, and other related fields. Our primary objective is to present research conducted through a cell biological perspective, addressing the essential mechanisms governing cell function, cellular interactions, and responses to the environment. Moreover, we focus on understanding the collective behavior of cells, culminating in the formation of tissues, organs, and whole organisms, while also investigating the consequences of any malfunctions in these intricate processes.