The presence of human polyomavirus JC (JCPyV) in pediatric brain tumors: a plausible trigger in Wnt/β-catenin pathway.

IF 1.9 4区 医学 Q3 NEUROSCIENCES
Sara Passerini, Sara Messina, Marta De Angelis, Lucia Nencioni, Francesca Gianno, Manila Antonelli, Valeria Pietropaolo
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引用次数: 0

Abstract

JC polyomavirus (JCPyV) is associated with progressive multifocal leukoencephalopathy (PML), but its plausible role in brain cancers is also disputed. One candidate to mediate cell transformation is the Large T antigen (LTAg), which has the capability to bind the Wnt pathway protein β-catenin, thus deregulating the cell cycle. In the current study, we investigated the presence and molecular state of JCPyV in pediatric brain tumors and the effects of virus-positivity on the Wnt pathway. JCPyV DNA was found in 31/101 (30.7%) brain tumors with a viral load of 3.2 copies/cell. The amplified NCCR revealed an archetype sequence, and VP1 reported a high degree of homology with the reference strain. The LTAg gene was reported in all JCPyV-positive tumors. Interestingly, among them, 5 tissues did not express VP1 and viral miRNAs, supporting a hampering of late region transcription. Over-expression of β-catenin, c-myc and cyclin D1 was observed in JCPyV-positive tissues compared to negative ones, suggesting that the virus may exploit this signaling pathway, potentially contributing to brain carcinogenesis. The current study adds further evidence of JCPyV prevalence in human brain tumors and reports alterations of the Wnt pathway, laying the basis for further investigation on JCPyV-mediated oncogenesis in the brain.

儿童脑肿瘤中存在人多瘤病毒JC (JCPyV): Wnt/β-catenin通路的可能触发因素
JC多瘤病毒(JCPyV)与进行性多灶性白质脑病(PML)有关,但其在脑癌中的合理作用也存在争议。介导细胞转化的一个候选者是大T抗原(LTAg),它具有结合Wnt通路蛋白β-catenin的能力,从而解除细胞周期的调节。在本研究中,我们研究了JCPyV在儿童脑肿瘤中的存在和分子状态,以及病毒阳性对Wnt通路的影响。JCPyV DNA在31/101(30.7%)脑肿瘤中发现,病毒载量为3.2拷贝/细胞。扩增的NCCR显示了一个原型序列,VP1与参考菌株高度同源。LTAg基因在所有jcpyv阳性肿瘤中均有报道。有趣的是,其中有5个组织不表达VP1和病毒mirna,支持后期区转录的阻碍。与阴性组织相比,在jcpyv阳性组织中观察到β-catenin、c-myc和cyclin D1的过度表达,表明该病毒可能利用这一信号通路,可能参与脑癌变。本研究进一步证实了JCPyV在人类脑肿瘤中的流行,并报道了Wnt通路的改变,为进一步研究JCPyV介导的脑内肿瘤发生奠定了基础。
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来源期刊
Journal of NeuroVirology
Journal of NeuroVirology 医学-病毒学
CiteScore
6.60
自引率
3.10%
发文量
77
审稿时长
6-12 weeks
期刊介绍: The Journal of NeuroVirology (JNV) provides a unique platform for the publication of high-quality basic science and clinical studies on the molecular biology and pathogenesis of viral infections of the nervous system, and for reporting on the development of novel therapeutic strategies using neurotropic viral vectors. The Journal also emphasizes publication of non-viral infections that affect the central nervous system. The Journal publishes original research articles, reviews, case reports, coverage of various scientific meetings, along with supplements and special issues on selected subjects. The Journal is currently accepting submissions of original work from the following basic and clinical research areas: Aging & Neurodegeneration, Apoptosis, CNS Signal Transduction, Emerging CNS Infections, Molecular Virology, Neural-Immune Interaction, Novel Diagnostics, Novel Therapeutics, Stem Cell Biology, Transmissable Encephalopathies/Prion, Vaccine Development, Viral Genomics, Viral Neurooncology, Viral Neurochemistry, Viral Neuroimmunology, Viral Neuropharmacology.
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