Plasmon-Enhanced Fluorescence Determination of Tamsulosin Using Silver Nanoparticles: A Green Analytical Strategy.

IF 3.1 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2025-09-17 DOI:10.1007/s10895-025-04545-y

Mariam Rashad, Fawzi Elsebaei, Zeinab Awad Sheribah, Mohamed Ibrahim Walash

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Abstract

A novel, simple, and highly sensitive spectrofluorometric method was developed for the quantification of Tamsulosin (TAM) in methanol, utilizing silver nanoparticles (AgNPs) as fluorescence enhancers. The AgNPs formed colloidal dispersions with particle sizes ranging between 5 and 50 nm, enhancing TAM's native fluorescence through a catalytic interaction. Fluorescence emission was measured at 315 nm after excitation at 285 nm, and the method exhibited linearity in the range of 100-500 ng/ml. The method was successfully applied to the assay of two commercial TAM capsule formulations obtained from a local pharmacy. Validation was carried out in accordance with International Council for Harmonisation (ICH) guidelines, confirming the method's accuracy, precision, and robustness. Furthermore, the ecological impact of the proposed method was evaluated using greenness assessment tools, including AGREE and the Analytical Eco-Scale, demonstrating its sustainability in terms of solvent use, chemical consumption, energy efficiency, and waste reduction.

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等离子体增强荧光测定纳米银坦索罗辛：一种绿色分析策略。

利用纳米银作为荧光增强剂，建立了一种新的、简单的、高灵敏度的甲醇中坦索罗辛（TAM）的荧光定量方法。AgNPs形成了粒径在5 ~ 50 nm之间的胶体分散体，通过催化相互作用增强了TAM的天然荧光。在285 nm处激发后，在315 nm处测量荧光发射，该方法在100-500 ng/ml范围内呈线性。该方法成功地应用于从当地药房获得的两种商业TAM胶囊配方的测定。按照国际协调委员会（ICH）指南进行验证，确认该方法的准确性、精密度和稳健性。此外，使用绿色评估工具（包括AGREE和Analytical Eco-Scale）对所提出方法的生态影响进行了评估，证明了其在溶剂使用、化学品消耗、能源效率和废物减少方面的可持续性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.