Antibacterial and antibiofilm activity of ruthenium polypyridine complex against Staphylococcus strains.

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-09-17 DOI:10.1080/08927014.2025.2558627

Leonardo Silva de Sousa, Alexandre Lopes Andrade, Aryane de Azevedo Pinheiro, Felipe Diógenes Abreu, Eduardo Henrique Silva Sousa, Idalina Maria Moreira de Carvalho, Débora S C M Castelo-Branco, Mayron Alves de Vasconcelos, Edson Holanda Teixeira

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引用次数: 0

Abstract

This study reports the synthesis of a new polypyridine ruthenium(II) complex, [Ru(anth)₃]²⁺, which generates singlet oxygen (Φ_Δ = 0.98) and binds DNA (K_b = 9.8 × 10⁴ M^-1), leading to bacterial damage. The compound exhibited minimum inhibitory concentrations (MICs) of 31.25 µg mL^-1 against S. aureus ATCC 700698 and S. epidermidis ATCC 35984, and 125 µg mL^-1 against S. aureus ATCC 25923 and S. epidermidis ATCC 12228. The compound showed synergistic effects with ampicillin and additive effects with tetracycline. The complex significantly reduced biofilm biomass, viable cell counts, and metabolic activity. Scanning electron and confocal laser microscopy confirmed surface disruption and reduced viability. Gel electrophoresis indicated light-induced DNA photocleavage. Cytotoxicity in L929 fibroblasts was observed only above 125 µg mL^-1. These findings suggest that [Ru(anth)₃]²⁺ is a promising antimicrobial agent with multi-target activity, supporting its potential for antimicrobial drug development.

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多吡啶钌络合物对葡萄球菌的抑菌和抗生物膜活性。

本研究报道了一种新的多吡啶钌（II）配合物[Ru(anth)3]2+的合成，该配合物产生单线态氧（ΦΔ = 0.98）并结合DNA (Kb = 9.8 × 104 M-1)，导致细菌损伤。该化合物对金黄色葡萄球菌ATCC 700698和表皮葡萄球菌ATCC 35984的最低抑制浓度为31.25µg mL-1，对金黄色葡萄球菌ATCC 25923和表皮葡萄球菌ATCC 12228的最低抑制浓度为125µg mL-1。该化合物与氨苄西林有协同作用，与四环素有加性作用。该复合物显著降低了生物膜生物量、活细胞计数和代谢活性。扫描电子和共聚焦激光显微镜证实了表面破坏和生存能力降低。凝胶电泳显示光诱导DNA光分裂。L929成纤维细胞的细胞毒性仅高于125µg mL-1。这些发现表明[Ru(anth)3]2+是一种具有多靶点活性的有前景的抗菌药物，支持其在抗菌药物开发中的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.