Antimycobacterial Activity of Allium Cepa and Allium sativum Hydroethanolic Crude Extracts against Pathogenic and Nonpathogenic Mycobacterial Strains.

IF 1.5 Q4 INFECTIOUS DISEASES
International Journal of Mycobacteriology Pub Date : 2025-07-01 Epub Date: 2025-09-15 DOI:10.4103/ijmy.ijmy_81_25
Joseph Mwanzia Nguta
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引用次数: 0

Abstract

Background: Tuberculosis (TB) caused by Mycobacterium tuberculosis complex remains a leading cause of morbidity and mortality worldwide. The zoonotic infectious condition represents a never-ending challenge toward which drug discovery efforts are needed. The current study was designed to evaluate the in vitro antimycobacterial activity of hydroethanolic extracts from Allium sativum and Allium cepa bulbs and leaves, traditionally used against respiratory tract illnesses, including TB.

Methods: The phenotypic colorimetric microplate Alamar blue assay was used to study the antimycobacterial activity of the ethanolic extracts against six mycobacterial strains. Each experiment was run in triplicate. Data generated were analyzed using descriptive statistics to obtain mean minimum inhibitory concentration (MIC) values.

Results: The A. sativum bulbs, A. sativum leaves, A. cepa bulbs, and A. cepa leaves exhibited MIC values of 19.5 µg/mL, 78.1 µg/mL, 78.1 µg/mL, and 19.5 µg/mL against the pathogenic mycobacterial strain, M. tuberculosis H37Rv (ATCC 27294), respectively.

Conclusion: In conclusion, the tested A. sativum and A. cepa bulbs and leaves have demonstrated significant activity against the pathogenic M. tuberculosis strain. This observation validates the ethnopharmacological use of the Allium species against TB. Further studies are required to isolate, elucidate the structure, and characterize the antimycobacterial compounds responsible for the observed activity. These will potentially contribute toward bioprospecting for a new class of ligands with activity against sensitive and drug-resistant strains of M. tuberculosis.

大蒜和大蒜氢乙醇粗提物对致病性和非致病性分枝杆菌的抑菌活性。
背景:结核分枝杆菌复合体引起的结核病(TB)仍然是世界范围内发病率和死亡率的主要原因。人畜共患传染病代表了一个永无止境的挑战,需要药物发现的努力。目前的研究旨在评估Allium sativum和Allium cepa球茎和叶子的氢乙醇提取物的体外抑菌活性,这两种物质传统上用于治疗包括结核病在内的呼吸道疾病。方法:采用表型比色微孔板Alamar蓝法研究乙醇提取物对6株分枝杆菌的抑菌活性。每个实验都做了三遍。使用描述性统计分析生成的数据以获得平均最小抑制浓度(MIC)值。结果:对结核分枝杆菌H37Rv (ATCC 27294)的MIC值分别为19.5µg/mL、78.1µg/mL、78.1µg/mL和19.5µg/mL。结论:本实验结果表明,sativum和cepa的球茎和叶片对致病性结核分枝杆菌具有较强的抗结核活性。这一观察结果证实了葱属植物抗结核的民族药理学作用。进一步的研究需要分离,阐明结构,并表征抗细菌化合物负责观察到的活性。这将有助于对一类具有抗敏感和耐药结核分枝杆菌活性的新型配体进行生物勘探。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.20
自引率
25.00%
发文量
62
审稿时长
7 weeks
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