Assessment of Expression LncRNA-xloc-000303, lncRNA-LOC152578 and hsa-miR-29a in Patients Diagnosed With Colon Cancer in the Pre-Treatment Stage

IF 1.9 Q4 ONCOLOGY

Cancer reports Pub Date : 2025-09-15 DOI:10.1002/cnr2.70321

Elham Tahami, Abolfazl Akbari, Reza Nekouian

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Abstract

Background

Employing a panel of biomarkers may enable the early diagnosis of colorectal cancer. Among these biomarkers, long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) are significant due to their crucial roles in biological and metabolic processes.

Aims

Therefore, the objective of this research was to evaluate the diagnostic value of the biomarkers LncRNA-xloc-000303, lncRNA-LOC152578, and miR-29a in the pre-treatment stage of colorectal cancer in Iranian patients.

Materials and Methods

In this study, 30 tumor tissue samples and 30 adjacent healthy tissue samples were collected, and RNA was extracted using the Trizol kit. Subsequently, the relative expression of the candidate microRNA and LncRNAs was analyzed using real-time PCR. The obtained gene expression data were then analyzed using REST software.

Results

In this study, it was found that the expression of LncRNA-xloc-000303 in tumor tissues significantly increased compared to healthy tissue (p < 0.001). Additionally, the analysis revealed that the expression level of lncRNA-LOC152578 showed an increase in tumor tissues compared to healthy tissues (p = 0.57), although these changes were not statistically significant. The results of the hsa-miR-29a expression analysis indicated a down regulation in tumor tissues compared to healthy tissues; however, this change was also not statistically significant (p = 0.34).

Conclusion

The results indicated that the expression changes of LncRNA-xloc-000303 in cancer tissue were significant compared to adjacent healthy tissue, suggesting its potential use as a biomarker for diagnosing colorectal cancer. However, further studies are needed to evaluate the biomarker specificity of lncRNA-LOC152578 and hsa-miR-29a. This study was conducted in an Iranian population and its results can be used in the future for personalized medicine in the prevention and treatment of colorectal cancer.

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LncRNA-xloc-000303、lncRNA-LOC152578和hsa-miR-29a在治疗前期结肠癌患者中的表达评估

背景：使用一组生物标志物可能有助于结直肠癌的早期诊断。在这些生物标志物中，长链非编码rna （LncRNAs）和微rna （miRNAs）因其在生物和代谢过程中的重要作用而具有重要意义。因此，本研究的目的是评估LncRNA-xloc-000303、lncRNA-LOC152578和miR-29a在伊朗结直肠癌患者治疗前阶段的诊断价值。材料与方法：本研究采集30例肿瘤组织样本和30例相邻健康组织样本，采用Trizol试剂盒提取RNA。随后，使用real-time PCR分析候选microRNA和lncrna的相对表达量。然后使用REST软件分析获得的基因表达数据。结果：本研究发现LncRNA-xloc-000303在肿瘤组织中的表达较健康组织显著升高(p)。结论：结果提示LncRNA-xloc-000303在癌组织中的表达变化较邻近健康组织显著，提示其有可能作为结直肠癌诊断的生物标志物。然而，lncRNA-LOC152578和hsa-miR-29a的生物标志物特异性需要进一步的研究来评估。这项研究是在伊朗人群中进行的，其结果可以在未来用于预防和治疗结直肠癌的个性化医疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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