Evaluating the efficacy of non-activated microbubble emulsions in biofilm removal from 3D-printed root canals.

IF 3.1 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Investigations Pub Date : 2025-09-16 DOI:10.1007/s00784-025-06554-4

Ahmet Keles, Anil Kishen

{"title":"Evaluating the efficacy of non-activated microbubble emulsions in biofilm removal from 3D-printed root canals.","authors":"Ahmet Keles, Anil Kishen","doi":"10.1007/s00784-025-06554-4","DOIUrl":null,"url":null,"abstract":"Objectives: This study evaluates the effectiveness of non-activated microbubble emulsions in removing biofilms in simulated minimally shaped root canals.Materials and methods: Enterococcus faecalis biofilms were developed over three weeks in various sizes of 3D-printed root canal models. The biofilm removal efficiency of microbubble emulsions (MB) was compared with several irrigants, sodium hypochlorite (NaOCl), distilled water (DW), and hydrogen peroxide (HP), across different root canal dimensions (20.04, 20.06, 40.04, 40.06). Irrigation was performed using 2.5 mL of each solution for one minute. In the MB groups, the canal was filled with a single emulsion drop and left for one minute. We used confocal laser scanning microscopy and crystal violet colorimetric analysis to assess the effectiveness of biofilm eradication. Bonferroni tests and two-way ANOVA were employed to analyze the data.Results: In narrow canals, MB emulsions demonstrated biofilm removal efficacy comparable to NaOCl (P > .05). However, NaOCl was the most effective irrigation solution for wider canals (P < .05). Canal size significantly influenced the biofilm removal efficacy in the MB group (P < .05). Overall, both MB emulsions and NaOCl achieved better biofilm removal results than DW and HP (P < .05).Conclusion: The non-activated MBs have shown effective antibiofilm properties in narrow canals, making them suitable for a minimally invasive approach and indicating their potential role in developing improved irrigation protocols.Clinical relevance: This study highlights the potential of microbubble emulsions as effective irrigants for minimally shaped root canals, supporting minimally invasive endodontic strategies.","PeriodicalId":10461,"journal":{"name":"Clinical Oral Investigations","volume":"29 10","pages":"452"},"PeriodicalIF":3.1000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Oral Investigations","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00784-025-06554-4","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: This study evaluates the effectiveness of non-activated microbubble emulsions in removing biofilms in simulated minimally shaped root canals.

Materials and methods: Enterococcus faecalis biofilms were developed over three weeks in various sizes of 3D-printed root canal models. The biofilm removal efficiency of microbubble emulsions (MB) was compared with several irrigants, sodium hypochlorite (NaOCl), distilled water (DW), and hydrogen peroxide (HP), across different root canal dimensions (20.04, 20.06, 40.04, 40.06). Irrigation was performed using 2.5 mL of each solution for one minute. In the MB groups, the canal was filled with a single emulsion drop and left for one minute. We used confocal laser scanning microscopy and crystal violet colorimetric analysis to assess the effectiveness of biofilm eradication. Bonferroni tests and two-way ANOVA were employed to analyze the data.

Results: In narrow canals, MB emulsions demonstrated biofilm removal efficacy comparable to NaOCl (P > .05). However, NaOCl was the most effective irrigation solution for wider canals (P < .05). Canal size significantly influenced the biofilm removal efficacy in the MB group (P < .05). Overall, both MB emulsions and NaOCl achieved better biofilm removal results than DW and HP (P < .05).

Conclusion: The non-activated MBs have shown effective antibiofilm properties in narrow canals, making them suitable for a minimally invasive approach and indicating their potential role in developing improved irrigation protocols.

Clinical relevance: This study highlights the potential of microbubble emulsions as effective irrigants for minimally shaped root canals, supporting minimally invasive endodontic strategies.

查看原文本刊更多论文

评价非活化微泡乳剂去除3d打印根管生物膜的效果。

目的：本研究评估非活化微泡乳剂去除模拟最小形状根管中生物膜的有效性。材料与方法：粪肠球菌生物膜在三周内制成各种尺寸的3d打印根管模型。比较了微泡乳剂（MB）与次氯酸钠（NaOCl）、蒸馏水（DW）和双氧水（HP）在不同根管尺寸（20.04、20.06、40.04、40.06）下的生物膜去除效果。用每种溶液2.5 mL灌洗1分钟。在MB组中，用单滴乳剂填充管并放置一分钟。我们使用激光共聚焦扫描显微镜和结晶紫比色分析来评估生物膜根除的有效性。采用Bonferroni检验和双向方差分析对数据进行分析。结果：在狭窄的管道中，MB乳剂的生物膜去除效果与NaOCl相当（P < 0.05）。结论：非活化的MBs在狭窄的管道中显示出有效的抗菌膜特性，使其适合微创入路，并表明其在开发改进的灌溉方案中具有潜在的作用。临床意义：本研究强调了微泡乳剂作为微创根管有效冲洗剂的潜力，支持微创根管治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical Oral Investigations 医学-牙科与口腔外科

CiteScore

6.30

自引率

5.90%

发文量

484

审稿时长

3 months

期刊介绍： The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.