Identification and Antimicrobial Potential of Marine Sponges (Carteriospongia foliascens, Callyspongia fallax, and Paratetilla arcifera) from Kenyan Marine Waters.

IF 3.2 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2025-09-03 eCollection Date: 2025-01-01 DOI:10.1155/ijm/4208163
Teresia Nyambura Wacira, Huxley Mae Makonde, Joseph Nyingi Kamau, Cromwell Mwiti Kibiti
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引用次数: 0

Abstract

Emerging and re-emerging infectious diseases and pathogens present a significant global public health threat that has led researchers to focus on discovering new antimicrobial agents in order to address the challenge. Sponges are a promising source of marine natural products, which can be used as lead molecules for drug discovery. This study was aimed at identifying marine sponges through morphological and molecular techniques and evaluate the bioactivity potential of their organic crude extracts against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Deoxyribonucleic acid (DNA) barcoding of the cytochrome c oxidase subunit I (COI) gene identified three genera of sponges (Carteriospongia, Callyspongia, and Paratetilla). Disk diffusion assay was used to determine the inhibition zone diameter (IZD) of the sponges' extracts. Minimum inhibitory concentrations (MICs) and the minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the most active sponge extracts were determined. The bioactive compounds were analyzed using gas chromatography-mass spectrometry (GC-MS). The dichloromethane extracts of Carteriospongia foliascens demonstrated the highest antifungal activity against C. albicans (31.33 ± 1.2 mg mL-1), surpassing the standard drug fluconazole (29.33 ± 1.5 mg mL-1). The MIC values for the sponge extracts ranged from 3.86 to 5.89 mg mL-1, and the ethyl acetate extract of Callyspongia fallax had an MBC of 4.03 mg mL-1 against S. aureus. GC-MS chromatogram identified 98 compounds across 41 classes in three sponge extracts. Notably, 9.2% of these compounds have been reported to exhibit antimicrobial activity against human pathogens. This study confirms that sponges are a source of useful biochemicals, which have potential for drug discovery. To the best of our knowledge, this is the first comprehensive study to report on the characterization of marine sponges from the Kenyan waters. Further research work to structurally elucidate and identify the most active bioactive compounds from the extracts of C. foliascens and C. fallax is recommended.

Abstract Image

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肯尼亚海域海绵体(叶状斑海绵、黄斑斑海绵和叶面海绵)的鉴定和抗菌潜力。
新出现和再出现的传染病和病原体对全球公共卫生构成重大威胁,这促使研究人员集中精力发现新的抗菌剂,以应对这一挑战。海绵是一种很有前途的海洋天然产物来源,可以作为药物开发的先导分子。本研究旨在通过形态学和分子技术鉴定海洋海绵,并评价其有机粗提物对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的生物活性潜力。细胞色素c氧化酶亚基I (COI)基因的脱氧核糖核酸(DNA)条形码鉴定了三属海绵(Carteriospongia, Callyspongia和Paratetilla)。采用圆盘扩散法测定海绵提取物的抑菌带直径(IZD)。测定了最有效海绵提取物的最低抑菌浓度(mic)和最低杀菌/杀真菌浓度(MBCs/ mfc)。采用气相色谱-质谱联用(GC-MS)对活性成分进行分析。对白色念珠菌的抑菌活性最高(31.33±1.2 mg mL-1),超过标准药物氟康唑(29.33±1.5 mg mL-1)。海绵提取物对金黄色葡萄球菌的MIC值在3.86 ~ 5.89 mg mL-1之间,而黄斑Callyspongia fallax乙酸乙酯提取物对金黄色葡萄球菌的MIC值为4.03 mg mL-1。GC-MS图谱鉴定出3种海绵提取物共41类98种化合物。值得注意的是,据报道,这些化合物中有9.2%对人类病原体具有抗菌活性。这项研究证实了海绵是一种有用的生物化学物质的来源,具有开发药物的潜力。据我们所知,这是第一个关于肯尼亚水域海绵特征的综合研究报告。建议开展进一步的研究工作,以从结构上阐明和鉴定出叶菖蒲和黄菖蒲提取物中最具活性的生物活性化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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