SPIN.DOC induces cellular transformation of NIH3T3 normal mouse fibroblast cells.

IF 1 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI:10.22099/mbrc.2025.52821.2125

Khuraijam Mrinalini Devi, Thangjam Davis Singh, Rubismita Deka, Lisam Shanjukumar Singh, Thiyam Ramsing Singh

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Abstract

SPIN.DOC was discovered as a transcriptional co-repressor of wingless related integration site (WNT) pathway. However, it has been found to be upregulated in various types of cancer, including hepatocellular carcinoma, colorectal cancer, renal papillary cell carcinoma. Whether SPIN.DOC functions as an oncogene or tumour suppressor gene remains uncertain. Here, we report that ectopic expression of SPIN.DOC in normal NIH3T3 fibroblast cells promotes cell proliferation, colony formation, migration and invasion. Moreover, SPIN.DOC expressing NIH3T3 cells show increased spheroid formation, suggesting enhanced stemness and transformation potential. Immunofluorescence analysis using anti-β-Tubulin suggests that SPIN.DOC may induce, formation of tubulin-based microtentacles (McTNs), indicating epithelial-to-mesenchymal (EMT) transition. In conclusion, our study helps in establishing that SPIN.DOC can function as an oncogene.

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SPIN.DOC诱导NIH3T3正常小鼠成纤维细胞的细胞转化。

doc被发现是无翅相关整合位点（WNT）通路的转录共抑制因子。然而，它已被发现在各种类型的癌症中上调，包括肝细胞癌、结直肠癌、肾乳头状细胞癌。至于SPIN.DOC是作为癌基因还是肿瘤抑制基因，目前尚不清楚。在这里，我们报道了正常NIH3T3成纤维细胞中SPIN.DOC的异位表达促进了细胞增殖、集落形成、迁移和侵袭。此外，表达NIH3T3细胞的SPIN.DOC显示球状体形成增加，表明细胞的干性和转化潜力增强。利用抗β-微管蛋白的免疫荧光分析表明，自旋doc可能诱导微管蛋白微触手（McTNs）的形成，表明上皮细胞向间质细胞（EMT）的转变。总之，我们的研究有助于确立SPIN.DOC作为致癌基因的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.