Identification of the PP1 interactome in human dental pulp: Insights for regenerative strategies

IF 3.7 2区生物学 Q2 CELL BIOLOGY

Cellular signalling Pub Date : 2025-09-12 DOI:10.1016/j.cellsig.2025.112132

Anna Kobrock , Daniela Patrício , Bárbara Matos , Eduardo Zarzuela , Javier Muñoz , John Howl , Pedro S. Gomes , Margarida Fardilha

{"title":"Identification of the PP1 interactome in human dental pulp: Insights for regenerative strategies","authors":"Anna Kobrock , Daniela Patrício , Bárbara Matos , Eduardo Zarzuela , Javier Muñoz , John Howl , Pedro S. Gomes , Margarida Fardilha","doi":"10.1016/j.cellsig.2025.112132","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Dental caries are one of the most prevalent chronic diseases worldwide affecting all age groups. Current therapeutic strategies are technically demanding and invasive procedures that only address the restoration of dental pulp. Upon damage, mineralized tissues and dental pulp are affected, activating signalling cascades that promote their repair and regeneration through stem cells differentiation into odontoblasts-like cells. Protein phosphatase 1 (PP1) participates in a panoply of cellular events, including those related with regenerative outcomes, such as odontogenic differentiation, highlighting the potential of its modulation.</div></div><div><h3>Methods</h3><div>Here we evaluated the expression of PP1 isoforms in dental pulp cells lines. We also characterized the PP1 interactome in dental pulp and identified potential interactors involved in repair and regenerative processes.</div></div><div><h3>Results</h3><div>The expression of all PP1 isoforms (α, β and γ) was detected in human dental cell lines. Moreover, a total of 258 proteins were identified as PP1γ regulatory interactors of protein phosphatase 1 (RIPPOs) in dental pulp, through a combination of a bioinformatic and co-immunoprecipitation/mass spectrometry analyses. Among these proteins, RRBP1 and THBS1 are promising targets, due to their roles in modulation of the regenerative response and in odontoblasts differentiation and mineralization.</div></div><div><h3>Conclusion</h3><div>This is the first reported PP1 interactome in dental pulp that identifies potential targets to modulate PP1 activity and potentially promote dental pulp regeneration.</div></div>","PeriodicalId":9902,"journal":{"name":"Cellular signalling","volume":"136 ","pages":"Article 112132"},"PeriodicalIF":3.7000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular signalling","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0898656825005479","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Dental caries are one of the most prevalent chronic diseases worldwide affecting all age groups. Current therapeutic strategies are technically demanding and invasive procedures that only address the restoration of dental pulp. Upon damage, mineralized tissues and dental pulp are affected, activating signalling cascades that promote their repair and regeneration through stem cells differentiation into odontoblasts-like cells. Protein phosphatase 1 (PP1) participates in a panoply of cellular events, including those related with regenerative outcomes, such as odontogenic differentiation, highlighting the potential of its modulation.

Methods

Here we evaluated the expression of PP1 isoforms in dental pulp cells lines. We also characterized the PP1 interactome in dental pulp and identified potential interactors involved in repair and regenerative processes.

Results

The expression of all PP1 isoforms (α, β and γ) was detected in human dental cell lines. Moreover, a total of 258 proteins were identified as PP1γ regulatory interactors of protein phosphatase 1 (RIPPOs) in dental pulp, through a combination of a bioinformatic and co-immunoprecipitation/mass spectrometry analyses. Among these proteins, RRBP1 and THBS1 are promising targets, due to their roles in modulation of the regenerative response and in odontoblasts differentiation and mineralization.

Conclusion

This is the first reported PP1 interactome in dental pulp that identifies potential targets to modulate PP1 activity and potentially promote dental pulp regeneration.

Abstract Image

查看原文本刊更多论文

人类牙髓中PP1相互作用组的鉴定：再生策略的见解。

背景：龋齿是影响所有年龄组的最普遍的慢性疾病之一。目前的治疗策略是技术上的要求和侵入性的程序，只解决牙髓的恢复。损伤后，矿化组织和牙髓受到影响，激活信号级联反应，通过干细胞分化为成牙细胞样细胞促进其修复和再生。蛋白磷酸酶1 （PP1）参与了一系列细胞事件，包括那些与再生结果相关的事件，如牙源性分化，突出了其调节的潜力。方法：观察PP1亚型在牙髓细胞系中的表达。我们还描述了牙髓中PP1相互作用组的特征，并确定了参与修复和再生过程的潜在相互作用。结果：PP1在人牙细胞系中均有表达（α、β、γ）。此外，通过生物信息学和免疫沉淀/质谱联合分析，共鉴定出258种蛋白是牙髓中蛋白磷酸酶1 （RIPPOs）的PP1γ调节相互作用因子。在这些蛋白中，RRBP1和THBS1是有希望的靶点，因为它们在调节再生反应和成牙细胞分化和矿化中起作用。结论：这是首次报道的牙髓中PP1相互作用体，它确定了调节PP1活性并可能促进牙髓再生的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cellular signalling 生物-细胞生物学

CiteScore

8.40

自引率

0.00%

发文量

250

审稿时长

27 days

期刊介绍： Cellular Signalling publishes original research describing fundamental and clinical findings on the mechanisms, actions and structural components of cellular signalling systems in vitro and in vivo. Cellular Signalling aims at full length research papers defining signalling systems ranging from microorganisms to cells, tissues and higher organisms.