Correlation between LC-MS/MS and ELISA methods for quantitative analysis of desmosine-containing solutions.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-09-14 DOI:10.1080/17576180.2025.2557182

Arisa Araki, Mahiro Takano, Christian Nanga Chick, Takumi Yamazaki, Jun Nojima, Toyonobu Usuki

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引用次数: 0

Abstract

Background: Desmosine and isodesmosine are crosslinking amino acids found in extracellular matrix protein elastin, which imparts elasticity to tissues such as those of the lungs and arteries. These compounds are promising biomarkers for diseases involving elastin degradation, such as chronic obstructive pulmonary disease.

Research design and method: This study examined the correlation between isotope-dilution LC-MS/MS and a newly established ELISA for in vitro diagnosis using a variety of samples.

Results: Results of ELISA and LC-MS/MS analyses exhibited a high correlation coefficient (0.9941). However, whereas the LC-MS/MS measurements deviated approximately 2-fold from the theoretical values, the ELISA measurements ranged from 0.83 to 1.06 (avg 0.94) times the theoretical values. Precise measurement of the absorbance of synthetic desmosine revealed a molar extinction coefficient of 2403, which differed markedly from the previously reported value of 4900 in 1963. Using this value to recalculate the amount of added desmosine, the LC-MS/MS measurements were 0.68 to 0.99 (avg 0.87) times the theoretical values.

Conclusion: Thus, the developed ELISA enables highly accurate determination of desmosine concentrations, comparable to LC-MS/MS, suggesting that ELISA is a potentially useful in vitro diagnostic tool.

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LC-MS/MS与ELISA法定量分析含去糖肽溶液的相关性研究。

背景：桥桥苷和异桥桥苷是在细胞外基质蛋白弹性蛋白中发现的交联氨基酸，它赋予组织（如肺和动脉）弹性。这些化合物是有前途的生物标志物，涉及弹性蛋白降解疾病，如慢性阻塞性肺疾病。研究设计与方法：本研究考察了同位素稀释LC-MS/MS与新建立的用于多种样品体外诊断的ELISA之间的相关性。结果：ELISA与LC-MS/MS分析结果具有较高的相关系数（0.9941）。然而，LC-MS/MS测量值与理论值相差约2倍，而ELISA测量值的范围为理论值的0.83至1.06倍（平均0.94倍）。对合成氨基葡萄糖吸光度的精确测量显示其摩尔消光系数为2403，与1963年报道的4900有明显差异。使用此值重新计算添加的氨基葡萄糖量，LC-MS/MS测量值为理论值的0.68 ~ 0.99（平均0.87）倍。结论：所建立的酶联免疫吸附试验（ELISA）可与LC-MS/MS相媲美，具有较高的准确性，是一种潜在的有用的体外诊断工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.